| Imaging local neuronal activity by monitoring PO(2) transients in capillaries. | |
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MedLine Citation:
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PMID: 23314058 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Two-photon phosphorescence lifetime microscopy (2PLM) has been used recently for depth measurements of oxygen partial pressure (PO(2)) in the rodent brain. In capillaries of olfactory bulb glomeruli, 2PLM has also allowed simultaneous measurements of PO(2) and blood flow and revealed the presence of erythrocyte-associated transients (EATs), which are PO(2) gradients that are associated with individual erythrocytes. We investigated the extent to which EAT properties in capillaries report local neuronal activity. We find that at rest, PO(2) at EAT peaks overestimates the mean PO(2) by 35 mm Hg. PO(2) between two EAT peaks is at equilibrium with, and thus reports, PO(2) in the neuropil. During odor stimulation, there is a small PO(2) decrease before functional hyperemia, showing that the initial dip in PO(2) is present at the level of capillaries. We conclude that imaging oxygen dynamics in capillaries provides a unique and noninvasive approach to map neuronal activity. |
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Authors:
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Alexandre Parpaleix; Yannick Goulam Houssen; Serge Charpak |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-13 |
Journal Detail:
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Title: Nature medicine Volume: - ISSN: 1546-170X ISO Abbreviation: Nat. Med. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9502015 Medline TA: Nat Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1] Institut National de la Santé et de la Recherche Médicale (INSERM) U603, Paris, France. [2] Centre National de la Recherche Scientifique (CNRS), UMR 8154, Paris, France. [3] Laboratory of Neurophysiology and New Microscopies, Université Paris Descartes, Paris, France. |
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