Document Detail

Imaging local neuronal activity by monitoring PO₂ transients in capillaries.
MedLine Citation:
PMID:  23314058     Owner:  NLM     Status:  MEDLINE    
Two-photon phosphorescence lifetime microscopy (2PLM) has been used recently for depth measurements of oxygen partial pressure (PO(2)) in the rodent brain. In capillaries of olfactory bulb glomeruli, 2PLM has also allowed simultaneous measurements of PO(2) and blood flow and revealed the presence of erythrocyte-associated transients (EATs), which are PO(2) gradients that are associated with individual erythrocytes. We investigated the extent to which EAT properties in capillaries report local neuronal activity. We find that at rest, PO(2) at EAT peaks overestimates the mean PO(2) by 35 mm Hg. PO(2) between two EAT peaks is at equilibrium with, and thus reports, PO(2) in the neuropil. During odor stimulation, there is a small PO(2) decrease before functional hyperemia, showing that the initial dip in PO(2) is present at the level of capillaries. We conclude that imaging oxygen dynamics in capillaries provides a unique and noninvasive approach to map neuronal activity.
Alexandre Parpaleix; Yannick Goulam Houssen; Serge Charpak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-13
Journal Detail:
Title:  Nature medicine     Volume:  19     ISSN:  1546-170X     ISO Abbreviation:  Nat. Med.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-07     Completed Date:  2013-04-17     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  9502015     Medline TA:  Nat Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  241-6     Citation Subset:  IM    
Institut National de Santé et de Recherche Médicale (INSERM) U603, Paris, France.
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MeSH Terms
Capillaries / metabolism*
Erythrocytes / metabolism
Mice, Inbred C57BL
Microscopy, Fluorescence, Multiphoton
Neurons / physiology*
Oxygen / blood*
Partial Pressure
Rats, Wistar
Reg. No./Substance:

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