Document Detail

Imaging light-modulated release of synaptic vesicles in the intact retina: retinal physiology at the dawn of the post-electrode era.
MedLine Citation:
PMID:  16185743     Owner:  NLM     Status:  MEDLINE    
Here, we illustrate an optical method for directly measuring the light-regulated synaptic output of neurons in the retina. The method allows simultaneous recording from many retinal neurons in intact flat-mount preparations of the vertebrate retina. These recordings depend on the use of FM1-43, an activity-dependent fluorescent dye that selectively labels synaptic vesicles. Release of the dye, which occurs upon vesicle exocytosis, is detected with 2-photon microscopy. This utilizes an infrared laser to trigger fluorescence excitation of the dye, while minimally perturbing retinal activity by activating phototransduction in rods and cones. Using this approach, one can measure activity of single neurons in the intact retinal network and populations of neurons in different layers of the retina, providing a new way to examine the function of retinal synapses and how visual information is processed.
Sue-Yeon Choi; Zejuan Sheng; Richard H Kramer
Related Documents :
8930973 - Corticofugal feedback improves the timing of retino-geniculate signal transmission.
1756803 - Direct and indirect retinal input into degenerated dorsal lateral geniculate nucleus af...
20193723 - Glia: the many ways to modulate synaptic plasticity.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2005-09-23
Journal Detail:
Title:  Vision research     Volume:  45     ISSN:  0042-6989     ISO Abbreviation:  Vision Res.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-05     Completed Date:  2006-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417402     Medline TA:  Vision Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  3487-95     Citation Subset:  IM    
Department of Molecular and Cell Biology, University of California, Berkeley, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Fluorescent Dyes
Microscopy, Electron
Neurons / metabolism,  ultrastructure
Pyridinium Compounds / metabolism*
Quaternary Ammonium Compounds / metabolism*
Retina / metabolism,  ultrastructure*
Synapses / metabolism,  physiology
Synaptic Transmission / physiology
Synaptic Vesicles / metabolism,  physiology*,  ultrastructure
Reg. No./Substance:
0/FM1 43; 0/Fluorescent Dyes; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Studies using a viral challenge and CD8 T cell depletions on the roles of cellular and humoral immun...
Next Document:  Electrochemical dechlorination of chloroacetic acids (CAAs) using hemoglobin-loaded carbon nanotube ...