Document Detail

Imaging the lateral diffusion of membrane molecules with quantum dots.
MedLine Citation:
PMID:  17406518     Owner:  NLM     Status:  MEDLINE    
This protocol describes a sensitive approach to tracking the motion of membrane molecules such as lipids and proteins with molecular resolution in live cells. This technique makes use of fluorescent semiconductor nanocrystals, quantum dots (QDs), as a probe to detect membrane molecules of interest. The photostability and brightness of QDs allow them to be tracked at a single particle level for longer periods than previous fluorophores, such as fluorescent proteins and organic dyes. QDs are bound to the extracellular part of the object to be followed, and their movements can be recorded with a fluorescence microscope equipped with a spectral lamp and a sensitive cooled charge-coupled device camera. The experimental procedure described for neurons below takes about 45 min. This technique is applicable to various cultured cells.
Hiroko Bannai; Sabine Lévi; Claude Schweizer; Maxime Dahan; Antoine Triller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature protocols     Volume:  1     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2006  
Date Detail:
Created Date:  2007-04-04     Completed Date:  2007-11-05     Revised Date:  2008-03-24    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  2628-34     Citation Subset:  IM    
INSERM U789, Biologie Cellulaire de la Synapse N&P, Ecole Normale Supérieure Paris, 46, Rue d'Ulm 75005 Paris, France.
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MeSH Terms
Cell Membrane / metabolism*
Membrane Lipids / metabolism
Membrane Proteins / metabolism
Microscopy, Fluorescence
Molecular Probe Techniques*
Quantum Dots*
Reg. No./Substance:
0/Membrane Lipids; 0/Membrane Proteins

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