| Imaging of intracellular acidic compartments with a sensitive rhodamine based fluorogenic pH sensor. | |
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MedLine Citation:
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PMID: 21792437 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The parameters of intracellular acidic compartments are altered in apoptosis, autophagy, cancer metastasis, etc. Low background and selective staining of lysosomes and autophagosomes was achieved with N-(rhodamine 6G)-lactam-ethylenediamine (R6G-EDA) which fluoresces in acidic vesicles via pH mediated ring opening of the intramolecular lactam. Long retention time of R6G-EDA in lysosomes and its exceptional stability against photo-bleaching allow full time tracking of lysosome morphology changes in tumor necrosis factor-α (TNF-α) triggered cell death, suggesting its utility for acidic vesicle studies in cell biology. |
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Authors:
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Zhu Li; Shuqi Wu; Jiahuai Han; Shoufa Han |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-27 |
Journal Detail:
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Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Chemical Biology, College of Chemistry and Chemical Engineering, and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, China. shoufa@xmu.edu.cn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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