Document Detail

Imaging of human mesenchymal stromal cells: homing to human brain tumors.
MedLine Citation:
PMID:  22081298     Owner:  NLM     Status:  MEDLINE    
Human mesenchymal stromal cells (hMSC) can be used as a drug delivery vehicle for the treatment of GBM. However, tracking the migration and distribution of these transplanted cells is necessary to interpret therapeutic efficacy. We compared three labeling techniques for their ability to track the migration of transplanted hMSC in an orthotopic mouse xenograft model. hMSC were labeled with three different imaging tags (fluorescence, luciferase or ferumoxide) for imaging by fluorescence, bioluminescence or magnetic resonance imaging (MRI), respectively. hMSC were labeled for all imaging modalities without the use of transfection agents. The labeling efficacy of the tags was confirmed, followed by in vitro and in vivo migration assays to track hMSC migration towards U87 glioma cells. Our results confirmed that the labeled hMSC retained their migratory ability in vitro, similar to unlabeled hMSC. In addition, labeled hMSC migrated towards the U87 tumor site, demonstrating their retention of tumor tropism. hMSC tumor tropism was confirmed by all three imaging modalities; however, MRI provides both real time assessment and the high resolution needed for clinical studies. Our findings suggest that ferumoxide labeling of hMSC is feasible, does not alter their migratory ability and allows detection by MRI. Non invasive tracking of transplanted therapeutic hMSC in the brain will allow further development of human cell based therapies.
Lata G Menon; John Pratt; Hong Wei Yang; Peter M Black; Gregory A Sorensen; Rona S Carroll
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-11-12
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  107     ISSN:  1573-7373     ISO Abbreviation:  J. Neurooncol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-07     Completed Date:  2012-07-09     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  257-67     Citation Subset:  IM    
Department of Neurosurgery, Brigham & Women's Hospital, Harvard Medical School, 221 Longwood Ave, Rm 121, Boston, MA 02115, USA.
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MeSH Terms
Brain Neoplasms / pathology*,  surgery,  ultrastructure
Cell Line, Tumor
Cell Movement / physiology
Cells, Cultured
Dextrans / diagnostic use
Disease Models, Animal
Glioblastoma / pathology*,  surgery,  ultrastructure
Iron / metabolism
Luciferases / diagnostic use
Magnetic Resonance Imaging
Magnetite Nanoparticles / diagnostic use
Mesenchymal Stem Cell Transplantation / methods
Mesenchymal Stromal Cells / physiology*,  ultrastructure
Mice, Nude
Microscopy, Electron, Transmission
Xenograft Model Antitumor Assays
Grant Support
Reg. No./Substance:
0/Magnetite Nanoparticles; 7439-89-6/Iron; 9004-54-0/Dextrans; EC 1.13.12.-/Luciferases; G6N3J05W84/ferumoxides

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