Document Detail


Imaging dynamic cell-cell junctional coupling in vivo using Trojan-LAMP.
MedLine Citation:
PMID:  19160519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study the physiological regulation and function of cell-cell gap junction communication in vivo, we developed a bioconjugate of caged dye, named dextran-CANPE-HCC, for imaging cell coupling in small model organisms. In vitro, the compound was photolyzed efficiently with robust fluorescence enhancement. Dextran-CANPE-HCC delivered into Caenorhabditis elegans oocytes was retained in cells throughout development. Using local uncaging, we photolyzed dextran-CANPE-HCC to release the small HCC dye and imaged the dynamics of intercellular dye transfer through gap junction channels, a technique we named Trojan-local activation of molecular fluorescent probes (LAMP). Early during embryonic development, the pattern of cell coupling undergoes dramatic remodeling and imaging revealed that the germ cell precursors, P2, P3 and P4, were isolated from the somatic cell communication compartment. As dextran-CANPE-HCC is chemically and metabolically stable, Labeled worms showed very bright signal upon photoactivation after hatching, which allowed us to examine cell coupling in living worms noninvasively.
Authors:
Yan-Ming Guo; Shiuhwei Chen; Premnath Shetty; Genhua Zheng; Rueyling Lin; Wen-hong Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature methods     Volume:  5     ISSN:  1548-7091     ISO Abbreviation:  Nat. Methods     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2009-01-22     Completed Date:  2009-02-05     Revised Date:  2013-03-28    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  835-41     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastomeres / metabolism
Caenorhabditis elegans
Cell Communication
Fluorescent Dyes*
Gap Junctions / physiology*
Microscopy, Fluorescence / methods*
Photolysis
Grant Support
ID/Acronym/Agency:
R01 GM077593/GM/NIGMS NIH HHS; R01 GM077593-05/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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