Document Detail

Imaging of the closed-chest mouse pulmonary circulation using synchrotron radiation microangiography.
MedLine Citation:
PMID:  21527665     Owner:  NLM     Status:  MEDLINE    
Structural and functional changes of pulmonary circulation related to pathophysiology of pulmonary arterial hypertension (PAH) remain to be fully elucidated. Angiographic visualization in in vivo animals provided a powerful tool for assessing the major indexes associated with the pathogenesis of PAH. In this study, we have exploited the full potential of synchrotron radiation (SR) microangiography to show the ability to visualize pulmonary hemodynamics in a closed-chest mouse. Male adult mice were anesthetized and cannulated with a customized 24-gauge catheter into the right ventricle via the jugular vein for administering iodine contrast agent. The microangiography was performed on the left lung. We measured dynamic changes in vessel diameter in response to acetylcholine (ACh) and acute exposure to hypoxic gas (10% O(2)). Moreover, the pulmonary transit time was estimated by the time of contrast agent circulating. We were able to visualize the pulmonary arteries from the left pulmonary artery (LPA) to the third generation of branching (inner diameter <100 μm). ACh and acute hypoxia induced vascular responses chiefly in the second and third branching vessels rather than the LPA and the first branching vessels. The transit time was only 0.83 s. These results demonstrate the effectiveness of SR for visualizing the pulmonary circulation in a closed-chest mouse. Future studies using SR microangiography on specific gene-targeted knockout and transgenic mice will provide new insights into the pathophysiology of pulmonary dysfunction and functional adaptation to survive in hypoxic condition.
Takashi Sonobe; Daryl O Schwenke; James T Pearson; Misa Yoshimoto; Yutaka Fujii; Keiji Umetani; Mikiyasu Shirai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-28
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  111     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-12     Completed Date:  2013-07-08     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM    
Department of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
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MeSH Terms
Acetylcholine / pharmacology
Angiography / methods*
Anoxia / physiopathology,  radiography
Blood Flow Velocity
Contrast Media / diagnostic use
Lung / blood supply*
Mice, Inbred C57BL
Microcirculation* / drug effects
Pulmonary Artery / drug effects,  radiography*
Pulmonary Circulation* / drug effects
Regional Blood Flow
Time Factors
Vasodilator Agents / pharmacology
Reg. No./Substance:
0/Contrast Media; 0/Vasodilator Agents; 51-84-3/Acetylcholine

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