Document Detail

Imaging the cellular response to transient shear stress using stroboscopic digital holography.
MedLine Citation:
PMID:  22191911     Owner:  NLM     Status:  In-Data-Review    
We use stroboscopic quantitative phase microscopy to study cell deformation and the response to cavitation bubbles and transient shear stress resulting from laser-induced breakdown of an optically trapped nanoparticle. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to cavitation induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laser-induced breakdown of a nanoparticle offers localized cavitation, which interacts with a single cell but without causing cell lysis.
Maciej Antkowiak; Yoshihiko Arita; Kishan Dholakia; Frank Gunn-Moore
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  16     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  120508     Citation Subset:  IM    
SUPA, University of St Andrews, School of Physics and Astronomy, St Andrews, Fife, KY16 9SS, ScotlandSULSA, University of St Andrews, School of Biology, St Andrews, Fife, KY16 9TF, Scotland.
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