Document Detail

Imaging of cardiac movement using ratiometric and nonratiometric optical mapping: effects of ischemia and 2, 3-butaneodione monoxime.
MedLine Citation:
PMID:  16398420     Owner:  NLM     Status:  MEDLINE    
Transmembrane voltage-sensitive fluorescent dyes are used to study electrical activity in hearts. Green and red fluorescence emissions from di-4-ANEPPS excited with 488 nm light indicate both transmembrane voltage changes and heart movement. We have previously shown that the ratio, green fluorescence divided by red fluorescence, indicates the transmembrane voltage without effects of movement. Here we examine the feasibility of measuring the movement, which is useful for the study of cardiac function, by subtracting this ratiometric signal from the red or green fluorescence signal. The results of this subtraction show tissue movement and its relative changes during cardiac ischemia and perfusion with an electromechanical uncoupling agent. By incorporating the spatial variations in fluorescence intensity from the heart, tissue movement can be qualitatively mapped to examine relative changes, however, with limited ability to quantify absolute displacement. Since these maps are obtained simultaneously with corresponding transmembrane potentials, the method allows study of spatiotemporal cardiac movement patterns and their relationship to the action potential.
Herman D Himel; Stephen B Knisley
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  IEEE transactions on medical imaging     Volume:  25     ISSN:  0278-0062     ISO Abbreviation:  IEEE Trans Med Imaging     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-09     Completed Date:  2006-02-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8310780     Medline TA:  IEEE Trans Med Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  122-7     Citation Subset:  IM    
Department of Biomedical Engineering of the School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
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MeSH Terms
Diacetyl / administration & dosage,  analogs & derivatives*
Feasibility Studies
Fluorescent Dyes
Heart Conduction System / drug effects,  physiopathology*
Image Interpretation, Computer-Assisted / methods
Microscopy, Fluorescence / methods*
Myocardial Contraction*
Myocardial Ischemia / complications,  diagnosis*,  physiopathology*
Spectrometry, Fluorescence / methods
Ventricular Dysfunction, Left / diagnosis,  physiopathology
Grant Support
52003//PHS HHS
Reg. No./Substance:
0/Fluorescent Dyes; 431-03-8/Diacetyl; 57-71-6/diacetylmonoxime

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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