| Imaging of acute heart-transplant rejection using 99m-Technetium labelled oligonucleotides against interleukin-2 mRNA in rats. | |
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MedLine Citation:
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PMID: 20116274 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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OBJECTIVES: Today, acute cardiac rejection is detected by endomyocardial biopsy, which harbours many risks. Thus, there is a necessity for less invasive methods. Since interleukin-2 (IL2) is over-expressed in acute graft rejection, we use radioactive DNA-fragments complementary to the mRNA of IL2 to detect graft rejection scintigraphically. METHODS: In a rat model of acute graft rejection, the oligonucleotide sequence complementary to the mRNA of IL2 is labelled with 99m-Technetium and injected intravenously. Scintigraphic and Geiger-counter activity of the transplants are evaluated and correlated with the current rejection classification of the International Society for Heart and Lung Transplantation (ISHLT). RESULTS: From the fourth postoperative day onwards, the scintigraphic images show a significant increase of radioactivity (p<0.05) in the rejected organs than in the accepted grafts. While scintigraphy is not significantly correlated with the standard rejections classification of the ISHLT, there is significant correlation between the ISHLT classification and radioactivity in the Geiger-counter analysis. CONCLUSIONS: Radioactively labelled anti-sense-oligonucleotides against mRNA of IL2 may be a promising approach for the detection of acute transplant rejection in vivo. |
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Authors:
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Mortimer Gierthmuehlen; Per Sommer; Maaz Zuhayra; Juergen Hedderich; Ute Jaenig; Fred Faendrich; Eberhard Henze; Willm Uwe Kampen |
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Publication Detail:
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Type: Journal Article Date: 2010-01-29 |
Journal Detail:
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Title: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery Volume: 37 ISSN: 1873-734X ISO Abbreviation: Eur J Cardiothorac Surg Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804069 Medline TA: Eur J Cardiothorac Surg Country: Germany |
Other Details:
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Languages: eng Pagination: 1111-6 Citation Subset: IM |
Copyright Information:
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Copyright 2009 European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Clinic for Nuclear Medicine, University of Schleswig Holstein, Campus Kiel, Germany. mortimer.gierthmuehlen@uniklinik-freiburg.de |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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