Document Detail


Imaging Myocardial Ischemia and Reperfusion Injury via Cy5.5-Annexin V.
MedLine Citation:
PMID:  24900054     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
AIM: The aim of this article is to present the results of an imaging study of myocardial apoptosis induced by ischemia/reperfusion injury.
METHODS: Twenty nude mice were randomly divided into an experimental group (10 mice) and control group (10 mice). In the experimental group, myocardial apoptosis was induced by ligation of the left anterior descending coronary artery (LAD) for 30 min. This was followed by reperfusion for 90 min. In the control group, the heart was exposed for the same length of time as in the experimental group. Cy5.5-annexin V (25 μg) was injected into both sets of mice after the onset of reperfusion. At 90 min post-injection, the mice were imaged. The region of interest (ROI) was obtained, and the fluorescence intensity of the ROI was quantified. The animals were sacrificed, and myocardial apoptosis was assayed by TUNEL assay.
RESULTS: Fluorescence intensity in the ischemia/reperfusion hearts was significantly higher than that in the control group (P < 0.05). In the TUNEL assay, more apoptotic cells were observed in the experimental group than in the control group, correlating with imaging results.
CONCLUSION: Fluorescence imaging of Cy5.5-annexin V in a mouse model of myocardial ischemia/reperfusion can be used in vivo as a noninvasive means of detecting ischemia/reperfusion-induced apoptotic cells in the heart.
Authors:
Rong Tian; DongFeng Pan
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Publication Detail:
Type:  Journal Article     Date:  2012-05-15
Journal Detail:
Title:  Nuclear medicine and molecular imaging     Volume:  46     ISSN:  1869-3474     ISO Abbreviation:  Nucl Med Mol Imaging     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2014-06-06     Completed Date:  2014-06-06     Revised Date:  2014-06-11    
Medline Journal Info:
Nlm Unique ID:  101530478     Medline TA:  Nucl Med Mol Imaging     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  155-61     Citation Subset:  -    
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