Document Detail

Imaging and Measuring the Molecular Force of Lymphoma Pathological Cells Using Atomic Force Microscopy.
MedLine Citation:
PMID:  22890585     Owner:  NLM     Status:  Publisher    
Atomic force microscopy (AFM) provides a new technology to visualize the cellular topography and quantify the molecular interactions at nanometer spatial resolution. In this work, AFM was used to image the cellular topography and measure the molecular force of pathological cells from B-cell lymphoma patients. After the fluorescence staining, cancer cells were recognized by their special morphological features and then the detailed topography was visualized by AFM imaging. The AFM images showed that cancer cells were much rougher than healthy cells. CD20 is a surface marker of B cells and rituximab is a monoclonal antibody against CD20. To measure the CD20-rituximab interaction forces, the polyethylene glycol (PEG) linker was used to link rituximab onto the AFM tip and the verification experiments of the functionalized probe indicated that rituximab molecules were successfully linked onto the AFM tip. The CD20-rituximab interaction forces were measured on about 20 pathological cells and the force measurement results indicated the CD20-rituximab binding forces were mainly in the range of 110-120 pN and 130-140 pN. These results can improve our understanding of the topography and molecular force of lymphoma pathological cells. SCANNING 00: 1-7, 2012. © 2012 Wiley Periodicals, Inc.
Mi Li; Xiubin Xiao; Lianqing Liu; Ning Xi; Yuechao Wang; Zaili Dong; Weijing Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-13
Journal Detail:
Title:  Scanning     Volume:  -     ISSN:  1932-8745     ISO Abbreviation:  Scanning     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903371     Medline TA:  Scanning     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© Wiley Periodicals, Inc.
State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China; Graduate University of Chinese Academy of Sciences, Beijing, China.
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