| Imaging mass spectrometry reveals unique lipid distribution in primary varicose veins. | |
| | |
MedLine Citation:
|
PMID: 20817502 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The lipid metabolism of varicose veins (VVs) remains unknown. To elucidate the pathogenesis of VV, we utilized the novel technique of imaging mass spectrometry (IMS). MATERIALS AND METHODS: We obtained VV tissues from 10 limbs of 10 VV patients who underwent great saphenous vein stripping. As control vein samples, we harvested segmental vein tissues from 6 limbs of 6 patients with peripheral artery occlusive disease who underwent infra-inguinal bypass with reversed saphenous vein grafting. To identify the localisation of lipid molecules in the VV tissues, we performed matrix-assisted laser desorption/ionization IMS (MALDI-IMS). We also performed MS/MS analyses to identify the structure of each molecule. RESULTS: We obtained mass spectra directly from control vein tissues and VV tissues and found a unique localisation of lipid molecules in the VV tissues. We localised lysophosphatidylcholine (LPC) (1-acyl 16:0), phosphatidylcholine (PC) (1-acyl 36:4) and sphingomyelin (SM) (d18:1/16:0) at the site of the VV valve. CONCLUSION: MALDI-IMS revealed the distribution of various lipid molecules in normal veins and VVs both. Accumulation of LPC (1-acyl 16:0), PC (1-acyl 36:4) and SM (d18:1/16:0) in the VV tissues suggested that inflammation associated with abnormal lipid metabolism may contribute to the development of VV. |
| | |
Authors:
|
H Tanaka; N Zaima; N Yamamoto; D Sagara; M Suzuki; M Nishiyama; Y Mano; M Sano; T Hayasaka; N Goto-Inoue; T Sasaki; H Konno; N Unno; M Setou |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery Volume: 40 ISSN: 1532-2165 ISO Abbreviation: Eur J Vasc Endovasc Surg Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-10-22 Completed Date: 2010-12-03 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9512728 Medline TA: Eur J Vasc Endovasc Surg Country: England |
Other Details:
|
Languages: eng Pagination: 657-63 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
|
Division of Vascular Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aged Aged, 80 and over Female Humans Lipid Metabolism / physiology Lipids* Male Mass Spectrometry Middle Aged Saphenous Vein / metabolism*, physiopathology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* Varicose Veins / metabolism*, physiopathology |
| Chemical | |
Reg. No./Substance:
|
0/Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Planar cell polarity signaling, cilia and polarized ciliary beating.
Next Document: Gypsum treated fly ash as a liner for waste disposal facilities.