| Imaging macromolecular interactions at an interface. | |
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MedLine Citation:
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PMID: 20085337 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Important physiological, pathological, and technological processes occur at continuous and dispersed phase interfaces. Understanding these processes is limited by inability to quantitate molecular events occurring at the interface. To provide a model-independent measurement of protein concentration and mobility at the interface, we employed confocal laser scanning microscopy (CLSM). Fluorescently labeled albumin and fibrinogen were studied singly, pairwise, and with a surfactant, Pluronic F-127, in aqueous droplets. CLSM enables measurement of molecular behaviors manifest as surface inhomogeneity and of biophysical quantities including partitioning between the bulk and the gas-liquid (GL) interface. We conclude that albumin and fibrinogen behave substantially differently at the GL interface, adsorption from multispecies solutions is fundamentally different than adsorption from solutions of single species, and surfactants can inhibit proteins from occupying the interface. |
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Authors:
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Joshua W Lampe; Zhengzheng Liao; Ivan J Dmochowski; Portonovo S Ayyaswamy; David M Eckmann |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: 26 ISSN: 1520-5827 ISO Abbreviation: Langmuir Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-09 Completed Date: 2010-04-26 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
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Languages: eng Pagination: 2452-9 Citation Subset: IM |
Affiliation:
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Center for Resuscitation Science, Department of Emergency Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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chemistry* Fibrinogen / chemistry* Macromolecular Substances / chemistry* Microscopy, Confocal Particle Size Poloxamer / chemistry Solutions Surface Properties |
| Grant Support | |
ID/Acronym/Agency:
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1S10RR021113/RR/NCRR NIH HHS; R01 GM083030/GM/NIGMS NIH HHS; R01 HL060230-10/HL/NHLBI NIH HHS; R01 HL067986-05A2/HL/NHLBI NIH HHS; R01 HL60230/HL/NHLBI NIH HHS; R01 HL67986/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Macromolecular Substances; 0/Solutions; 106392-12-5/Poloxamer; 9001-32-5/Fibrinogen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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