| Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots. | |
| | |
MedLine Citation:
|
PMID: 16724226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The internalization of a series of water-soluble CdSe/CdS quantum dots (QDs) stabilized by citrate, isocitrate, succinate, and malate by Escherichia coli is established by epifluorescence and confocal fluorescence scanning microscopy, fluorimetry, and UV-vis spectroscopy on whole and lysed bacterial cells. The organic-acid-stabilized QDs span a range in size from 3.8+/-1.1 to 6.0+/-2.4 nm with emission wavelengths from 540 to 630 nm. QDs of different sizes (i.e., 3.8-6 nm) can enter the bacterium and be detected on different fluorescence channels with little interference from other QDs as a result of the distinct emission profiles (i.e., 540-630 nm, respectively). Costaining QD-labeled E. coli with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) demonstrates that the QDs and DAPI are colocalized within E. coli, whereas costaining QD-labeled E. coli with membrane dye FM4-64 shows that the FM4-64 is localized in the outer bacterial membrane and that the QDs are inside. |
| | |
Authors:
|
Matthew D Hirschey; Yong-Jin Han; Galen D Stucky; Alison Butler |
Related Documents
:
|
17592616 - Enantioseparation of chiral tropa alkaloids by means of cyclodextrin-modified microemul... 18211376 - Encapsulation of nutraceutical monoterpenes in beta-cyclodextrin and modified starch. 16529766 - Studies of quenching and enhancement of fluorescence of methyl orange adsorbed on silve... 16509706 - Adsorption at the liquid-liquid interface in the biphasic rhodium catalyzed hydroformyl... 19031306 - Development of the primary bacterial microfouling layer on antifouling and fouling rele... 23629486 - Facile water-assisted synthesis of cuo nanourchins and their application as nonenzymati... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-05-25 |
Journal Detail:
|
Title: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry Volume: 11 ISSN: 0949-8257 ISO Abbreviation: J. Biol. Inorg. Chem. Publication Date: 2006 Jul |
Date Detail:
|
Created Date: 2006-06-22 Completed Date: 2006-08-30 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9616326 Medline TA: J Biol Inorg Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 663-9 Citation Subset: IM |
Affiliation:
|
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106-9510, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cadmium Compounds
/
metabolism* Escherichia coli / cytology*, metabolism Microscopy, Confocal Quantum Dots* Selenium Compounds / metabolism* Staining and Labeling / methods* Sulfides / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
GM38130/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cadmium Compounds; 0/Selenium Compounds; 0/Sulfides; 1306-23-6/cadmium sulfide; 1306-24-7/cadmium selenide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The expression pattern of genes involved in early neurogenesis suggests distinct and conserved funct...
Next Document: Modeling proline ligation in the heme-dependent CO sensor, CooA, using small-molecule analogs.