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Imaging Cytoplasmic Lipid Droplets in Enterocytes and Assessing Dietary Fat Absorption.
MedLine Citation:
PMID:  24099292     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The primary function of the small intestine is digesting and absorbing nutrients from consumed food. Because of this, the small intestine is often thought of as a nutrient thoroughfare-enterocytes taking up nutrients on the apical side and then secreting nutrients from the basolateral side. The small intestine is not commonly thought of as a lipid storage organ; however, when meals and diets containing high amounts of fat are consumed, some dietary fat is stored in cytoplasmic lipid droplets (CLDs). The balance between storage and secretion of dietary fat by enterocytes is important in determining the physiological fate of dietary fat, including regulating blood lipid concentrations and energy balance. The existence of CLDs within enterocytes has likely evolved for three important physiological functions: (i) to allow the small intestine to efficiently absorb large amounts of energy dense fat, (ii) to control the rate of dietary fat entering circulation, and (iii) to alleviate lipotoxicity to enterocytes induced by high concentrations of free fatty acids, especially when a high fat meal is consumed. The purpose of this chapter is to provide methods for imaging CLDs in enterocytes and assessing different aspects of dietary fat absorption.
Authors:
Aki Uchida; Hyeon Jeong Lee; Ji-Xin Cheng; Kimberly K Buhman
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in cell biology     Volume:  116C     ISSN:  0091-679X     ISO Abbreviation:  Methods Cell Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373334     Medline TA:  Methods Cell Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  151-166     Citation Subset:  -    
Copyright Information:
© 2013 Elsevier Inc. All rights reserved.
Affiliation:
Department of Nutrition Science, Purdue University, West Lafayette, Indiana, USA; Interdisciplinary Life Science Program, Purdue University, West Lafayette, Indiana, USA.
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