Document Detail


Image-based analysis of light-grown seedling hypocotyls in Arabidopsis.
MedLine Citation:
PMID:  22893281     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Time-resolved hypocotyl length measurements in seedlings have the potential to greatly aid genetic studies looking at light, hormone, and circadian regulation of cell expansion. Recently, several computer-based tools have been developed to quantify hypocotyl length during photomorphogenesis and early seedling development. Here we detail a method for quantifying Arabidopsis seedling hypocotyls in an image-based assay, focusing on light-grown seedlings responding to shade conditions.
Authors:
Benjamin J Cole; Joanne Chory
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  918     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-08-15     Completed Date:  2012-12-20     Revised Date:  2013-02-14    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA. chory@salk.edu
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / growth & development*,  radiation effects*
Hypocotyl / growth & development*,  radiation effects*
Image Processing, Computer-Assisted
Light*
Molecular Imaging / methods*
Time Factors
Grant Support
ID/Acronym/Agency:
R01 GM052413/GM/NIGMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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