Document Detail


Image analysis and mathematical correlation between cell number and diameter of colonies in soft agar as a measurement of growth in soft agar.
MedLine Citation:
PMID:  1980037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Automated image analysis was used to measure the colony diameter and estimate the cell number in colonies under conditions for anchorage-independent growth (AIG). The calculated values for the number of AIG cells in a colony of a diameter greater than or equal to 60 microns were decidedly different from the actual values obtained by either automated or manual cell counting. Like animal cells that exhibit AIG, these colonies demonstrating anchorage independence can be enumerated reproducibly. However, the assumption is incorrect that the number of cells (N) in a colony expressing AIG varies directly with a change in the diameter of a colony (d). Whether one counts the number of cells manually in a colony of a definite diameter or arbitrarily uses a diameter function of greater than or equal to 60 microns as a linear distance that indicates the presence of 50 or more cells in a colony, each procedure has its own built-in bias. In procedures that require quantitative data, the most reliable procedure is to standardize the values for diameter of colonies with cell numbers in colonies.
Authors:
J C Lewis; G E Milo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Teratogenesis, carcinogenesis, and mutagenesis     Volume:  10     ISSN:  0270-3211     ISO Abbreviation:  Teratog., Carcinog. Mutagen.     Publication Date:  1990  
Date Detail:
Created Date:  1991-02-13     Completed Date:  1991-02-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8100917     Medline TA:  Teratog Carcinog Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  351-7     Citation Subset:  IM    
Affiliation:
Human Nutrition and Food Management, Ohio State University, Columbus 43210.
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MeSH Terms
Descriptor/Qualifier:
Agar
Cell Division*
Cell Transformation, Neoplastic
Humans
Image Processing, Computer-Assisted*
Mathematics
Microscopy, Fluorescence
Reproducibility of Results
Spectrophotometry, Ultraviolet
Grant Support
ID/Acronym/Agency:
ES46023/ES/NIEHS NIH HHS; R01 CA25907/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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