Document Detail

Image analysis of the acetic acid-alcohol fixed metaphase chromosome.
MedLine Citation:
PMID:  7108996     Owner:  NLM     Status:  MEDLINE    
Electron micrographs of acetic acid-alcohol fixed, unstained human metaphase chromosomes have been analyzed with an image system. Electron micrographs were first digitized from over a million picture elements into a range of 64 gray levels which correspond to mass distribution. The obtained numerical data were then processed by programs which increased contrast and permitted enhancement and expansion of the original image. Despite the disruptive nature of such preparations, analysis of their TEM images reveals structural features previously reported from other preparative methods as well as morphological properties previously reported in metaphase chromosomes which required special pretreatments and staining for demonstration. Familial features included low density centromeres and secondary constrictions, higher density telomeres and paracentromeric regions, and G-band-like differential densities along chromatids. In addition, structural components were observed in a size range 25-56 nm. These masses appeared to have linear and circular forms oriented in perpendicular, diagonal and longitudinal arrays. They appeared in a nonrandom fashion and can be interpreted as demonstrating a relatively ordered chromosome.
P W Johnson; R I Barnett; E A Mackinnon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of submicroscopic cytology     Volume:  14     ISSN:  0022-4782     ISO Abbreviation:  J. Submicrosc. Cytol.     Publication Date:  1982 Jan 
Date Detail:
Created Date:  1982-10-21     Completed Date:  1982-10-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0235232     Medline TA:  J Submicrosc Cytol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  31-43     Citation Subset:  IM    
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MeSH Terms
Chromatids / ultrastructure
Chromosomes, Human / ultrastructure*
Microscopy, Electron

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