| Image Analysis of HER2 Immunohistochemical Staining: Reproducibility and Concordance With Fluorescence In Situ Hybridization of a Laboratory-Validated Scoring Technique. | |
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MedLine Citation:
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PMID: 22261453 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Image analysis of the HER2 immunohistochemical (IHC) stain can help determine which breast cancer patients may benefit from HER2-targeted therapy. We studied the concordance of HER2 IHC and fluorescence in situ hybridization (FISH) as well as reproducibility of surgical pathologist (SP) and cytotechnologist (CT) interpretations using manual and image analysis methodologies on 154 IHC cases. Concordances with FISH were good for IHC negative (0, 1+) cases (range, 97%-100%) and positive (3+) cases (range, 87%-100%). Image analysis had fewer equivocal (2+) results (10.4%) than CT (14.9%) and SP (16.2%) manual methods, with higher concordances to FISH (31%, 26%, and 20% for image analysis, CT manual, and SP manual, respectively). CT manual (κ = 0.747) and image analysis (κ = 0.779) methods had better interobserver reproducibility than SP manual (κ = 0.697). CT image analysis had better intraobserver reproducibility (κ = 0.882) than CT (κ = 0.828) and SP (κ = 0.766) manual methods. HER2 IHC analysis performed by image analysis can produce accurate results with improved reproducibility. |
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Authors:
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Douglas M Minot; Jesse Voss; Susan Rademacher; Toe Lwin; Jessica Orsulak; Bolette Caron; Rhett Ketterling; Aziza Nassar; Beiyun Chen; Amy Clayton |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 137 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 270-6 Citation Subset: AIM; IM |
Affiliation:
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Dept of Laboratory Medicine and Pathology, Mayo Clinic, 200 First St SW, Rochester, MN 55905. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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