| Iloprost has potent anti-inflammatory properties on human monocyte-derived dendritic cells. | |
| | |
MedLine Citation:
|
PMID: 20649610 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The stable prostaglandin I2 analogue (iloprost) iloprost has been shown to inhibit allergic airway inflammation in mice by modulating the function of myeloid dendritic cells (DCs). OBJECTIVE: The aim of the current study was to investigate the biological activity of iloprost on human monocyte-derived DCs. METHODS: I prostanoid (IP) receptor expression was analysed by RT-PCR. Cytokine secretion by DCs and CD4+ T cells was measured by ELISA. The expression of the transcription factor FoxP3 after co-culture of DCs with CD4+ CD45RA+ T cells was analysed by flow cytometry. RESULTS: Human monocyte-derived DCs were found to express mRNA specific for the PGI2 receptor IP, and stimulation with iloprost resulted in increased cyclic AMP levels in both immature DCs (iDCs) and mature DCs (mDCs). Moreover, iloprost dose dependently inhibited the secretion of TNF-alpha, IL-6, IL-8 and IL-12p70 in mDCs, while it enhanced IL-10 production. Changes in cytokine secretion were paralleled by an altered T-cell priming capacity of DCs: in co-culture experiments of iloprost-treated mDC and naïve CD45RA+ T cells, an induction of regulatory T cells could be observed, as demonstrated by increased intracellular FoxP3 expression and IL-10 production. Additionally, iloprost inhibited the MIP-3beta-induced migration of mDCs. CONCLUSION: In summary, our results provide evidence that iloprost profoundly affects the function of human myeloid DCs. Therefore, iloprost might also be a new therapeutical option for the treatment of asthma in humans. |
| | |
Authors:
|
T Müller; T Dürk; B Blumenthal; Y Herouy; S Sorichter; M Grimm; E Panther; S Cicko; J Norgauer; M Idzko |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 40 ISSN: 1365-2222 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-07-23 Completed Date: 2010-11-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
|
Languages: eng Pagination: 1214-21 Citation Subset: IM |
Affiliation:
|
Department of Pneumology, University of Freiburg, Freiburg, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Inflammatory Agents
/
pharmacology* Cell Separation Cytokines / biosynthesis Dendritic Cells / drug effects*, immunology, metabolism Enzyme-Linked Immunosorbent Assay Flow Cytometry Forkhead Transcription Factors / biosynthesis Humans Iloprost / pharmacology* Lymphocyte Activation / drug effects, immunology Monocytes / cytology, immunology, metabolism Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / drug effects, immunology, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Inflammatory Agents; 0/Cytokines; 0/FOXP3 protein, human; 0/Forkhead Transcription Factors; 78919-13-8/Iloprost |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Infections and asthma: new insights into old ideas.
Next Document: Transnasal transsphenoid approach: a minimally invasive approach for removal of cavernous haemangiom...