| Il-4 and Il-13 Inhibit Il-1β and Tnf-α Induced Kinin B(1) and B(2) Receptors Through a Stat6 Dependent Mechanism. | |
| | |
MedLine Citation:
|
PMID: 23351078 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND AND PURPOSE: Bone resorption induced by interleukin-1β (IL-1 β) and tumour necrosis factor (TNF-α) is synergistically potentiated by kinins, partially due to enhanced kinin receptor expression. Inflammation induced bone resorption can be impaired by IL-4 and IL-13. The aim was to investigate if expression of B(1) and B(2) kinin receptors can be affected by IL-4 and IL-13. EXPERIMENTAL APPROACH: We examined effects in a human osteoblastic cell line (MG-63), primary human gingival fibroblasts and mouse bones by IL-4 and IL-13 on mRNA and protein expression of the B(1) and B(2) kinin receptors. We also examined the role of STAT6 by RNA interference and using Stat6(-/-) mice. KEY RESULTS: IL-4 and IL-13 decreased the mRNA expression of B(1) and B(2) kinin receptors induced by either IL-1β or TNF-α in MG-63 cells, intact mouse calvarial bones or primary human gingival fibroblasts. The burst of intracellular calcium induced by either bradykinin (B(2) agonist) or des-Arg(10) -Lys-bradykinin (B(1) agonist) in gingival fibroblasts pretreated with IL-1β was impaired by IL-4. Similarly, the increased binding of B(1) and B(2) ligands induced by IL-1β was decreased by IL-4. In calvarial bones from Stat6 deficient mice, and in fibroblasts in which STAT6 was knocked down by siRNA, the effect of IL-4 was decreased. CONCLUSIONS AND IMPLICATIONS: These data show, for the first time, that IL-4 and IL-13 decrease kinin receptors in a STAT6-dependent mechanism, which can be one important mechanism by which these cytokines exert their anti-inflammatory effects and impair bone resorption. |
| | |
Authors:
|
P P C Souza; A B Brechter; R I Reis; C A S Costa; P Lundberg; U H Lerner |
Related Documents
:
|
16505488 - Endocytic function of von hippel-lindau tumor suppressor protein regulates surface loca... 18779368 - Receptor trafficking controls weak signal delivery: a strategy used by c-met for stat3 ... 11016818 - Receptor occupancy with digoxin vs receptor occupancy with a putative endogenous digita... 9824558 - Fibroblast growth factor receptors regulate the ability for hindlimb regeneration in xe... 20488168 - The mechanisms of zn2+ effects on ca2+-permeable ampa receptors on carp retinal horizon... 2971938 - Effects of monensin on the receptor-mediated endocytosis of 125i-labelled igg by guinea... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-28 |
Journal Detail:
|
Title: British journal of pharmacology Volume: - ISSN: 1476-5381 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2013 The Authors. British Journal of Pharmacology © 2013 The British Pharmacological Society. |
Affiliation:
|
Department of Molecular Periodontology, Umeå University, Umeå, Sweden; Department of Physiology and Pathology, Araraquara School of Dentistry,University Estadual Paulista, Araraquara, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mice lacking Period 1 and Period 2 circadian clock genes exhibit blue cone photoreceptor defects.
Next Document: Complete mitochondrial genome of Blue-crowned Parakeet (Aratinga acuticaudata)-phylogenetic position...