Document Detail


IgG1 and IgG4 are the predominant subclasses among auto-antibodies against two citrullinated antigens in RA.
MedLine Citation:
PMID:  18703528     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Antibody subclasses reflect specific immunological processes and may be indicative of the underlying pathological pattern in an autoimmune disease like RA. We therefore quantified anti-cyclic citrullinated peptides (CCP) and anti- citrullinated vimentin (MCV) IgG subclass titres in RA patients and compared them with the respective titres of antibodies directed against the varicella zoster virus (VZV) and to total serum titres. METHODS: Sera of 77 patients fulfilling the ACR criteria for RA were collected. An IgG subclass-specific ELISA system was then established and combined with commercially available MCV, CCP and VZV pre-coated microtitre plates. RESULTS: Even though IgG1 is the predominant subclass among antibodies against CCP and MCV in RA patients, IgG4 is second with respect to titres and frequencies. This increase in IgG4 among RA-specific antibodies is independent of disease duration and does not reflect a general skewing of the immune response in these patients as overall serum titres and antibodies directed against VZV show a normal distribution of IgG1, IgG2, IgG3 and IgG4. CONCLUSION: Elevated IgG4 titres are specific for auto-antibodies against citrullinated antigens in RA and are indicative of a Th2-biased environment during the generation of auto-reactive plasma cells. We discuss here an indirect role for IgG4 auto-antibodies in hindering the elimination of auto-reactive B and plasma cells and thus driving the autoimmune process.
Authors:
R Engelmann; J Brandt; M Eggert; K Karberg; A Krause; G Neeck; B Mueller-Hilke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-13
Journal Detail:
Title:  Rheumatology (Oxford, England)     Volume:  47     ISSN:  1462-0332     ISO Abbreviation:  Rheumatology (Oxford)     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2008-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883501     Medline TA:  Rheumatology (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1489-92     Citation Subset:  AIM; IM    
Affiliation:
Institute of Immunology, University of Rostock, Schillingallee 70, 18055 Rostock, Germany. robby.engelmann@med.uni-rostock.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Antibodies, Viral / blood
Arthritis, Rheumatoid / immunology*
Autoantibodies / blood*
Cohort Studies
Enzyme-Linked Immunosorbent Assay / methods
Herpesvirus 3, Human / immunology
Humans
Immunoglobulin G / blood*
Middle Aged
Peptides, Cyclic / immunology*
Vimentin / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Autoantibodies; 0/Immunoglobulin G; 0/Peptides, Cyclic; 0/Vimentin; 0/cyclic citrullinated peptide

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