| IgG anti-Galnac-GD1a antibodies bind to neuromuscular junctions of rat hemidiaphragm. | |
| | |
MedLine Citation:
|
PMID: 23055311 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Introduction: We investigated the localization of a ganglioside, N-acetylgalactosaminyl GD1a (GalNAc-GD1a), in peripheral nerves with an IgG anti-GalNAc-GD1a antibody, which was produced in rabbits immunized with GalNAc-GD1a. Methods: Teased fibers from ventral and dorsal roots and hemidiaphragm sections of rats were assessed using fluorescent double- and triple-labeling methods. Results: The nodal and paranodal regions of teased fibers from ventral roots were immunostained with IgG anti-GalNAc-GD1a antibodies. After collagenase treatment, no staining was seen with IgG anti-GalNAc-GD1a or anti-NF200 antibodies, whereas α-bungarotoxin selectively stained nerve terminals. In cross-sectional and longitudinal sections of rat hemidiaphragm, IgG anti-GalNAc-GD1a antibodies overlapped with α-BuTx and anti-NF200 antibodies, indicating that GalNAc-GD1a is localized to the nerve terminal. IgG anti-GalNAc-GD1a antibody staining also overlapped with that of AChR clusters and syntaxin-positive presynaptic nerve terminals. Conclusion: GalNAc-GD1 is localized in both pre- and postsynaptic nerve terminals of neuromuscular junctions. Muscle Nerve 46: 705-710, 2012. |
| | |
Authors:
|
Takumi Nagaoka; Sayako Hotta; Terumasa Chiba; Iku Utsunomiya; Kenji Abe; Hiide Yoshino; Chiaki Koshikawa; Kyoji Taguchi |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Muscle & nerve Volume: 46 ISSN: 1097-4598 ISO Abbreviation: Muscle Nerve Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-10-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7803146 Medline TA: Muscle Nerve Country: United States |
Other Details:
|
Languages: eng Pagination: 705-10 Citation Subset: IM |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan; Department of Pharmaceuticals, Dokkyo University School of Medicine, Mibu, Tochigi, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ocular neuropathy in peripheral neuropathies.
Next Document: Measurement of cross-sectional area of cervical roots and brachial plexus trunks.