| IgG adsorption on a new protein A adsorbent based on macroporous hydrophilic polymers II. Pressure-flow curves and optimization for capture. | |
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MedLine Citation:
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PMID: 19786279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pressure-flow curves are obtained for a new protein A adsorbent matrix based on macroporous hydrophilic polymer beads with average diameter of 57 microm and a narrow particle size distribution. Experimental data are obtained in a 1cm diameter laboratory column and in preparative scale columns with diameters of 20, 30, and 45 cm. The results are consistent with a model that assumes a linear relationship between bed compression and relative flow velocity. Surprisingly, the packing compressibility is essentially independent of column diameter for the preparative columns. As a result, after accounting for the variation in extraparticle porosity caused by compression, the column pressure drop is accurately predictable using the Carman-Kozeny equation. A model is also developed to predict productivity for IgG capture as a function of operating conditions based on dynamic binding capacity data presented in Part I of this work. For typical conditions, the model predicts maximum productivity at low residence times, between 1 and 1.5 min, when the dynamic binding capacity is at about 70-80% of the maximum. Combining the two models for column pressure and for dynamic binding capacity allows the design of preparative scale columns that maximize productivity while meeting specified pressure constraints. |
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Authors:
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Ernie X Perez-Almodovar; Giorgio Carta |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-09-18 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1216 ISSN: 1873-3778 ISO Abbreviation: J Chromatogr A Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-02 Completed Date: 2010-02-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 8348-54 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, University of Virginia, Charlottesville, VA 22904-4741, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography
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instrumentation,
methods* Equipment Design Immunoglobulin G / chemistry*, metabolism Models, Chemical* Particle Size Polymers / chemistry* Porosity Pressure Recombinant Proteins / chemistry, metabolism Staphylococcal Protein A / chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; 0/Polymers; 0/Recombinant Proteins; 0/Staphylococcal Protein A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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