Document Detail

IgG adsorption on a new protein A adsorbent based on macroporous hydrophilic polymers II. Pressure-flow curves and optimization for capture.
MedLine Citation:
PMID:  19786279     Owner:  NLM     Status:  MEDLINE    
Pressure-flow curves are obtained for a new protein A adsorbent matrix based on macroporous hydrophilic polymer beads with average diameter of 57 microm and a narrow particle size distribution. Experimental data are obtained in a 1cm diameter laboratory column and in preparative scale columns with diameters of 20, 30, and 45 cm. The results are consistent with a model that assumes a linear relationship between bed compression and relative flow velocity. Surprisingly, the packing compressibility is essentially independent of column diameter for the preparative columns. As a result, after accounting for the variation in extraparticle porosity caused by compression, the column pressure drop is accurately predictable using the Carman-Kozeny equation. A model is also developed to predict productivity for IgG capture as a function of operating conditions based on dynamic binding capacity data presented in Part I of this work. For typical conditions, the model predicts maximum productivity at low residence times, between 1 and 1.5 min, when the dynamic binding capacity is at about 70-80% of the maximum. Combining the two models for column pressure and for dynamic binding capacity allows the design of preparative scale columns that maximize productivity while meeting specified pressure constraints.
Ernie X Perez-Almodovar; Giorgio Carta
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-09-18
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1216     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-02     Completed Date:  2010-02-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  8348-54     Citation Subset:  IM    
Department of Chemical Engineering, University of Virginia, Charlottesville, VA 22904-4741, USA.
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MeSH Terms
Chromatography / instrumentation,  methods*
Equipment Design
Immunoglobulin G / chemistry*,  metabolism
Models, Chemical*
Particle Size
Polymers / chemistry*
Recombinant Proteins / chemistry,  metabolism
Staphylococcal Protein A / chemistry*,  metabolism
Reg. No./Substance:
0/Immunoglobulin G; 0/Polymers; 0/Recombinant Proteins; 0/Staphylococcal Protein A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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