Document Detail

IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis.
MedLine Citation:
PMID:  20944989     Owner:  NLM     Status:  In-Process    
The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5% and a specificity of 97.4%. IgM-WB showed a sensitivity of 54.8% and a specificity of 94.7%. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5%. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants.
Anderson S Machado; Gláucia M Q Andrade; José N Januário; Matheus D Fernandes; Ana Carolina A V Carneiro; Mariângela Carneiro; Ericka V M Carellos; Roberta M C Romanelli; Daniel V Vasconcelos-Santos; Ricardo W A Vitor
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Memórias do Instituto Oswaldo Cruz     Volume:  105     ISSN:  1678-8060     ISO Abbreviation:  Mem. Inst. Oswaldo Cruz     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502619     Medline TA:  Mem Inst Oswaldo Cruz     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  757-61     Citation Subset:  IM    
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
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