Document Detail

The IgD-binding domain of the Moraxella IgD-binding protein MID (MID962-1200) activates human B cells in the presence of T cell cytokines.
MedLine Citation:
PMID:  16301327     Owner:  NLM     Status:  MEDLINE    
Moraxella catarrhalis immunoglobulin D (IgD)-binding protein (MID) is an outer membrane protein with specific affinity for soluble and cell-bound human IgD. Here, we demonstrate that mutated M. catarrhalis strains devoid of MID show a 75% decreased activation of human B cells as compared with wild-type bacteria. In contrast to MID-expressing Moraxella, the MID-deficient Moraxella mutants did not bind to human CD19+ IgD+ B cells. The smallest MID fragment with preserved IgD-binding capacity comprises 238 amino acids (MID(962-1200)). To prove the specificity of MID(962-1200) for IgD, a Chinese hamster ovary (CHO) cell line expressing membrane-anchored human IgD was manufactured. MID(962-1200) bound strongly to the recombinant IgD on CHO cells. Moreover, MID(962-1200) stimulated peripheral blood lymphocyte (PBL) proliferation 5- and 15-fold at 0.1 and 1.0 microg/ml, respectively. This activation could be blocked completely by antibodies directed against the CD40 ligand (CD154). MID(962-1200) also activated purified B cells in the presence of interleukin (IL)-2 or IL-4. An increased IL-6 production was seen after stimulation with MID(962-1200), as revealed by a human cytokine protein array. MID(962-1200) fused to green fluorescent protein (GFP) bound to human B cells and activated PBL to the same degree as MID(962-1200). Taken together, MID is the only IgD-binding protein in Moraxella. Furthermore, the novel T cell-independent antigen MID(962-1200) may, together with MID(962-1200)-GFP, be considered as promising reagents in the study of IgD-dependent B cell activation.
Therése Nordström; Johan Jendholm; Martin Samuelsson; Arne Forsgren; Kristian Riesbeck
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-21
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  79     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-30     Completed Date:  2006-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  319-29     Citation Subset:  IM    
Department of Laboratory Medicine, Malmö University Hospital, Lund University, SE-205 02, Malmö, Sweden.
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MeSH Terms
Adhesins, Bacterial / immunology*,  pharmacology
Antibodies / pharmacology
B-Lymphocytes / drug effects,  immunology*
CD40 Ligand / drug effects,  immunology
CHO Cells
Cell Membrane / immunology
Cell Proliferation / drug effects
Cells, Cultured
Cytokines / immunology,  pharmacology*
Green Fluorescent Proteins / immunology
Immunoglobulin D / immunology*
Interleukin-6 / biosynthesis
Moraxella (Branhamella) catarrhalis / immunology*
T-Lymphocytes / drug effects,  immunology*
Time Factors
Reg. No./Substance:
0/Adhesins, Bacterial; 0/Antibodies; 0/Cytokines; 0/IgD-binding protein MID, Moraxella catarrhalis; 0/Immunoglobulin D; 0/Interleukin-6; 147205-72-9/CD40 Ligand; 147336-22-9/Green Fluorescent Proteins

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