Document Detail

IgA-mediated clearance and tissue deposition of dinitrophenylated human serum albumin at various DNP:HSA ratios.
MedLine Citation:
PMID:  3185569     Owner:  NLM     Status:  MEDLINE    
Dinitrophenylated human serum albumin (DNP-HSA) with various DNP:HSA ratios was prepared by direct conjugation with dinitrobenzene sulfate or with a caproic acid spacer group (DNP-cap-HSA) using DNP-epsilon-amino-caproic acid N-hydroxy-succinimide ester. The substitution ratio and chemical linkage (presence of a spacer group) were shown to affect the degree of murine anti-DNP antibody binding to antigen, and hence the tissue deposition and efficiency of hepatobiliary transport. DNP-HSA (4.5:1), which poorly binds to IgA anti-DNP antibody, is inefficiently transported into mouse bile. In contrast, 9:1 DNP-cap-HSA readily forms complexes and is more effectively cleared by the hepatobiliary route. The IgA-mediated increase in liver deposition of DNP-cap-HSA (9:1) was found to be associated with an increase in antigen uptake by hepatocytes. In contrast, large complexes formed between DNP-HSA (49:1) and IgA anti-DNP antibody are taken up by nonparenchymal cells of the liver and thus are inefficiently transported into bile. These results suggest that the IgA-mediated uptake by phagocytic cells or hepatocytes of haptenated protein strongly depends on the degree of haptenation.
J M Epps; J O Phillips; J Mestecky
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular immunology     Volume:  25     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-12-06     Completed Date:  1988-12-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  731-8     Citation Subset:  IM    
Department of Microbiology and Medicine, University of Alabama, Birmingham 35294.
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MeSH Terms
Antigen-Antibody Complex / metabolism
Bile / immunology
Dinitrophenols / immunology,  metabolism*
Immunoglobulin A / immunology*
Liver / immunology
Serum Albumin / metabolism*
Spleen / immunology
Grant Support
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Dinitrophenols; 0/Immunoglobulin A; 0/Serum Albumin; 0/dinitrophenyl-human serum albumin conjugate

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