| Idiopathic pulmonary fibrosis: immunohistochemical analysis provides fresh insights into lung tissue remodelling with implications for novel prognostic markers. | |
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MedLine Citation:
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PMID: 22295148 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: This study explored the cellular and biological interrelationships involved in Idiopathic Pulmonary Fibrosis (IPF) lung tissue remodelling using immunohistochemical analysis. METHODS AND RESULTS: IPF and control lung tissues were examined for localisation of Epithelial Mesenchymal Transition (EMT), proliferation and growth factor markers assessing their relationship to key histological aberrations. E-cadherin was expressed in IPF and control (Alveolar type II) ATII cells (>75%). In IPF, mean expression of N-cadherin was scanty (<10%): however 4 cases demonstrated augmented expression in ATII cells correlating to histological disease status (Pearson correlation score 0.557). Twist was expressed within fibroblastic foci but not in ATII cells. Transforming Growth Factor- β (TGF-β) protein expression was significantly increased in IPF ATII cells with variable expression within fibroblastic foci. Antigen Ki-67 was observed within hyperplastic ATII cells but not in cells overlying foci. Collagen I and α-smooth muscle actin (α-SMA) were strongly expressed within fibroblastic foci (>75%); cytoplasmic collagen I in ATII cells was present in 3 IPF cases. IPF ATII cells demonstrated variable Surfactant Protein-C (SP-C). CONCLUSIONS: The pathogenesis of IPF is complex and involves multiple factors, possibly including EMT. Histological analysis suggests TGF-β-stimulated myofib rob lasts initiate a contractile response within established fibroblastic foci while proliferating ATII cells attempt to instigate alveolar epithelium repair. Marker expression (N-cadherin and Ki-67) correlation with histological disease activity (as reflected by fibroblastic foci extent) may emerge as future prognostic indicators for IPF. |
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Authors:
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Nicola J Lomas; Keira L Watts; Khondoker M Akram; Nicholas R Forsyth; Monica A Spiteri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-01-07 |
Journal Detail:
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Title: International journal of clinical and experimental pathology Volume: 5 ISSN: 1936-2625 ISO Abbreviation: Int J Clin Exp Pathol Publication Date: 2012 |
Date Detail:
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Created Date: 2012-02-01 Completed Date: 2012-05-24 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 101480565 Medline TA: Int J Clin Exp Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 58-71 Citation Subset: IM |
Affiliation:
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Department of Cellular Pathology, University Hospital of North Staffordshire, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Epithelial-Mesenchymal Transition
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physiology* Humans Idiopathic Pulmonary Fibrosis / metabolism*, pathology* Immunohistochemistry Prognosis |
| Comments/Corrections | |
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