Document Detail


Identity of the renin cell is mediated by cAMP and chromatin remodeling: an in vitro model for studying cell recruitment and plasticity.
MedLine Citation:
PMID:  18055510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The renin-angiotensin system (RAS) regulates blood pressure and fluid-electrolyte homeostasis. A key step in the RAS cascade is the regulation of renin synthesis and release by the kidney. We and others have shown that a major mechanism to control renin availability is the regulation of the number of cells capable of making renin. The kidney possesses a pool of cells, mainly in its vasculature but also in the glomeruli, capable of switching from smooth muscle to endocrine renin-producing cells when homeostasis is threatened. The molecular mechanisms governing the ability of these cells to turn the renin phenotype on and off have been very difficult to study in vivo. We, therefore, developed an in vitro model in which cells of the renin lineage are labeled with cyan fluorescent protein and cells actively making renin mRNA are labeled with yellow fluorescent protein. The model allowed us to determine that it is possible to culture cells of the renin lineage for numerous passages and that the memory to express the renin gene is maintained in culture and can be reenacted by cAMP and chromatin remodeling (histone H4 acetylation) at the cAMP-responsive element in the renin gene.
Authors:
Ellen Steward Pentz; Maria Luisa S Sequeira Lopez; Magali Cordaillat; R Ariel Gomez
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-11-30
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  294     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-12     Completed Date:  2008-03-31     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H699-707     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Lineage / physiology
Cells, Cultured
Chromatin / metabolism,  physiology*
Cyclic AMP / physiology*
Cyclic AMP Response Element-Binding Protein / physiology
Dealkylation
Green Fluorescent Proteins / metabolism
Histones / metabolism
Immunoprecipitation
Kidney / cytology*,  metabolism*
Luminescent Proteins / metabolism
Mice
Mice, Transgenic
Muscle, Smooth, Vascular / cytology,  metabolism
RNA / biosynthesis,  isolation & purification
Recruitment, Neurophysiological / physiology
Renin / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
DK-52612/DK/NIDDK NIH HHS; HL-66242/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Chromatin; 0/Cyan Fluorescent Protein; 0/Cyclic AMP Response Element-Binding Protein; 0/Histones; 0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; 63231-63-0/RNA; E0399OZS9N/Cyclic AMP; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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