Document Detail


Identity of malonyl and palmitoyl transferase of fatty acid synthetase from yeast. Functional interrelationships between the acyl transferases.
MedLine Citation:
PMID:  391556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Functional interrelationships between the acyl transferases of yeast fatty acid synthetase were investigated. In binding assays with synthetase modified by 5,5'-dithiobis(2-nitrobenzoic acid), 4--5 malonyl transferase entities per multienzyme complex molecule could be titrated. In the presence of palmitoyl-CoA these malonyl transferases were found inaccessible to malonyl-CoA, whereas the acetyl transferases were reactive towards acetyl-CoA. Between four and five palmitoyl transferase entities per synthetase equivalent were found reactive towards palmitoyl-CoA, the palmitoyl binding being inhibited by malonyl-CoA. Following palmitoyl binding the acetyl transferases were found towards acetyl-CoA. Substrate model assays were consistent with these data. It is concluded that malonyl and palmitoyl transferases are closely coupled enzyme components of the multienzyme complex which are fairly independent of the acetyl transferase entities. The molecular basis for the observed coupling will be given in the following paper.
Authors:
H Engeser; K Hübner; J Straub; F Lynen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  101     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1979 Nov 
Date Detail:
Created Date:  1980-03-17     Completed Date:  1980-03-17     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  407-12     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / metabolism*
Dithionitrobenzoic Acid / pharmacology
Fatty Acid Synthetase Complex / metabolism*
Kinetics
Malonates
Palmitic Acids
Protein Binding
Saccharomyces cerevisiae / enzymology*
Chemical
Reg. No./Substance:
0/Malonates; 0/Palmitic Acids; 69-78-3/Dithionitrobenzoic Acid; EC 2.3.-/Acyltransferases; EC 6.-/Fatty Acid Synthetase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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