| Identity of malonyl and palmitoyl transferase of fatty acid synthetase from yeast. Functional interrelationships between the acyl transferases. | |
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MedLine Citation:
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PMID: 391556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Functional interrelationships between the acyl transferases of yeast fatty acid synthetase were investigated. In binding assays with synthetase modified by 5,5'-dithiobis(2-nitrobenzoic acid), 4--5 malonyl transferase entities per multienzyme complex molecule could be titrated. In the presence of palmitoyl-CoA these malonyl transferases were found inaccessible to malonyl-CoA, whereas the acetyl transferases were reactive towards acetyl-CoA. Between four and five palmitoyl transferase entities per synthetase equivalent were found reactive towards palmitoyl-CoA, the palmitoyl binding being inhibited by malonyl-CoA. Following palmitoyl binding the acetyl transferases were found towards acetyl-CoA. Substrate model assays were consistent with these data. It is concluded that malonyl and palmitoyl transferases are closely coupled enzyme components of the multienzyme complex which are fairly independent of the acetyl transferase entities. The molecular basis for the observed coupling will be given in the following paper. |
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Authors:
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H Engeser; K Hübner; J Straub; F Lynen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 101 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1979 Nov |
Date Detail:
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Created Date: 1980-03-17 Completed Date: 1980-03-17 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 407-12 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyltransferases
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metabolism* Dithionitrobenzoic Acid / pharmacology Fatty Acid Synthetase Complex / metabolism* Kinetics Malonates Palmitic Acids Protein Binding Saccharomyces cerevisiae / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Malonates; 0/Palmitic Acids; 69-78-3/Dithionitrobenzoic Acid; EC 2.3.-/Acyltransferases; EC 6.-/Fatty Acid Synthetase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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