Document Detail


Identity of macromolecules present in the extracellular slime layer of Staphylococcus epidermidis.
MedLine Citation:
PMID:  7647114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The extracellular slime layer of Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with M(r) ranging from 120,000 to 35,000 (average M(r) (80,000) containing a polysaccharide covalently bound to a small peptide; c) a papain degradable macromolecule (molecular mass 60 kDa) bearing acidic carbohydrates covalently bound to protein; and c) two acidic polysaccharides strongly retained by the anion-exchange column; one polysaccharide is sulphated and has a molecular mass of 20 kDa; the other has a higher charge density and a molecular mass of 12.5 kDa. The results obtained clearly demonstrate the presence of discrete macromolecules in the extracellular material of slime-producing S epidermidis, the majority of which contain acidic carbohydrates, whose biological role remains to be elucidated.
Authors:
N K Karamanos; H S Panagiotopoulou; A Syrokou; C Frangides; A Hjerpe; G Dimitracopoulos; E D Anastassiou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimie     Volume:  77     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  1995  
Date Detail:
Created Date:  1995-09-28     Completed Date:  1995-09-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  217-24     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Patras, Greece.
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MeSH Terms
Descriptor/Qualifier:
Chemical Fractionation
Electrophoresis
Extracellular Space / chemistry
Humans
Staphylococcus epidermidis / chemistry*,  ultrastructure

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