Document Detail

Identity of hepatic membrane transport systems for bile salts, phalloidin, and antamanide by photoaffinity labeling.
MedLine Citation:
PMID:  6591186     Owner:  NLM     Status:  MEDLINE    
Phalloidin, a bicyclic heptapeptide, and antamanide, a monocyclic decapeptide from the poisonous mushroom Amanita phalloides, interact with bile-salt-binding polypeptides of the hepatocyte membrane, as demonstrated by photoaffinity labeling using the photolabile bile salt derivative 7,7,-azo-3 alpha, 12 alpha-dihydroxy-5 beta-cholan-24-oic acid, either unconjugated or taurine conjugated. With the photolabile derivatives of phalloidin, N-delta-(4-[(1-azi-2,2,2-trifluoroethyl) benzoyl]-beta-alanyl)-delta-aminophalloin, (N epsilon-[4-(1-azi-2,2,2-trifluoroethyl)benzoyl]lys6)-anta manide, the same membrane polypeptides with apparent MrS of 54,000 and 48,000 were labeled as with the photolabile derivatives of unconjugated and conjugated bile salts. The presence of bile salts decreased markedly the extent of labeling of these phalloidin- and antamanide-binding polypeptides. These results indicate that hepatic uptake systems for bile salts, phallotoxins, and the cycloamanide antamanide are identical, thus explaining the organotropism of phallotoxins.
T Wieland; M Nassal; W Kramer; G Fricker; U Bickel; G Kurz
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  81     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-10-03     Completed Date:  1984-10-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5232-6     Citation Subset:  IM    
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MeSH Terms
Affinity Labels / metabolism*
Bile Acids and Salts / metabolism*
Biological Transport
Carrier Proteins / isolation & purification,  metabolism*
Cell Membrane / metabolism
Hydroxysteroid Dehydrogenases*
Liver / metabolism*
Membrane Glycoproteins*
Oligopeptides / metabolism*
Peptides, Cyclic / metabolism*
Phalloidine / metabolism*
Rats, Inbred Strains
Reg. No./Substance:
0/Affinity Labels; 0/Bile Acids and Salts; 0/Carrier Proteins; 0/Membrane Glycoproteins; 0/Oligopeptides; 0/Peptides, Cyclic; 0/bile acid binding proteins; 16898-32-1/antamanide; 17466-45-4/Phalloidine; EC 1.1.-/Hydroxysteroid Dehydrogenases; EC 1.1.1.-/AKR1C2 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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