Document Detail


Identity, developmental restriction and reactivity of extralaminar cells capping mammalian neuromuscular junctions.
MedLine Citation:
PMID:  19001504     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuromuscular junctions (NMJs) are normally thought to comprise three major cell types: skeletal muscle fibres, motor neuron terminals and perisynaptic terminal Schwann cells. Here we studied a fourth population of junctional cells in mice and rats, revealed using a novel cytoskeletal antibody (2166). These cells lie outside the synaptic basal lamina but form caps over NMJs during postnatal development. NMJ-capping cells also bound rPH, HM-24, CD34 antibodies and cholera toxin B subunit. Bromodeoxyuridine incorporation indicated activation, proliferation and spread of NMJ-capping cells following denervation in adults, in advance of terminal Schwann cell sprouting. The NMJ-capping cell reaction coincided with expression of tenascin-C but was independent of this molecule because capping cells also dispersed after denervation in tenascin-C-null mutant mice. NMJ-capping cells also dispersed after local paralysis with botulinum toxin and in atrophic muscles of transgenic R6/2 mice. We conclude that NMJ-capping cells (proposed name 'kranocytes') represent a neglected, canonical cellular constituent of neuromuscular junctions where they could play a permissive role in synaptic regeneration.
Authors:
Felipe A Court; Thomas H Gillingwater; Shona Melrose; Diane L Sherman; Kay N Greenshields; A Jennifer Morton; John B Harris; Hugh J Willison; Richard R Ribchester
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-11
Journal Detail:
Title:  Journal of cell science     Volume:  121     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  3901-11     Citation Subset:  IM    
Affiliation:
Euan MacDonald Centre for Motor Neurone Disease Research, The University of Edinburgh, 1 George Square, Edinburgh EH8 9JZ, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Basement Membrane / cytology,  ultrastructure
Cell Proliferation
Cells, Cultured
Immunohistochemistry
Mice
Mice, Inbred C57BL
Motor Neurons / metabolism
Neuromuscular Junction / cytology*,  metabolism,  ultrastructure
Presynaptic Terminals / metabolism
Rats
Rats, Sprague-Dawley
Schwann Cells / cytology,  metabolism,  ultrastructure
Grant Support
ID/Acronym/Agency:
//Medical Research Council; //Wellcome Trust

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