Document Detail


Identifying a site for maximum delivery of oxygen to transplanted cells.
MedLine Citation:
PMID:  10941217     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For in vivo cell implantation techniques to be successful, the energy and metabolic substrate requirement of the cells being grown must be met. Certain cells with high-energy requirements (e.g., hepatocytes, pancreatic island cells) experience a high degree of cell death after implantation due to a limited supply of oxygen. We proposed that the pleural cavity might be an oxygen-rich environment and hence an excellent site for cell implantation. To test the hypothesis that the delivery of oxygen to the pleural cavity is directly proportional to the inspired oxygen concentration we measured the pO(2) of saline instilled in the pleural cavity as compared to that of the peritoneal cavity. We postulated that the physiologic basis for any difference was the result of direct diffusion of oxygen into the pleural space across the alveoli. The study was conducted on sheep (n = 6), after induction of general anesthesia, in two phases, control and experimental. Saline was instilled into the peritoneal and pleural cavities via catheters, after equilibration at given FiO(2), the pO(2) of the paline aspirated from the two cavities was compared. In the experimental group, animals were sacrificed (no circulation) and ventilated. The same sequence of steps as in the control phase were repeated. In the control group, the pO(2) of saline aspirated from the pleural cavity approached the arterial pO(2) at all FiO(2) levels. The pO(2) of the peritoneal saline aspirate fell over time. In the experimental phase (no circulation), the pO(2) of the pleural cavity saline rose to >400 mm Hg. We conclude that this is a result of direct diffusion and is a potential source of unlimited oxygen supply not dependent on vascular supply.
Authors:
S Kaur; J Cortiella; C A Vacanti
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Tissue engineering     Volume:  6     ISSN:  1076-3279     ISO Abbreviation:  Tissue Eng.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-09-07     Completed Date:  2000-09-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9505538     Medline TA:  Tissue Eng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  229-32     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, UMass Memorial Medical Center, Worcester, Massachusetts, USA. shubjeet.Kaur@umassmed.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Transplantation*
Islets of Langerhans Transplantation*
Liver Transplantation*
Oxygen / metabolism*
Oxygen Consumption
Peritoneal Cavity*
Pleura*
Sheep
Chemical
Reg. No./Substance:
7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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