Document Detail


Identifying the origin of single corneal cells by DNA fingerprinting: part I--implications for corneal limbal allografting.
MedLine Citation:
PMID:  11333329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To demonstrate that the combination of impression cytology and single cell DNA fingerprinting represents a powerful tool that is suitable for detecting transplanted cells after corneal limbal allografting. METHODS: Fifty single cells were obtained by corneal impression cytology from 12 patients undergoing cataract surgery. Individual cells were isolated from samples by micromanipulation. Polymerase chain reaction and short tandem repeat profiling was used to obtain forensic standard "DNA fingerprints" from single cells. Blood samples taken at the time of impression cytology provided control "fingerprints." RESULTS: Informative DNA fingerprints were obtained from all corneal samples and 66% (33 of 50 cells) of isolated single cells. Of all fingerprints obtained, most (91%, 30 of 33 fingerprints) corneal fingerprints matched corresponding blood sample fingerprints. At least one corneal fingerprint matched the corresponding blood sample fingerprint in 83% (10 of 12 patients) of the patients in the study. CONCLUSIONS: This extremely specific single cell DNA fingerprinting system permits accurate identification of individual corneal epithelial cells, allowing very reliable determination of their origin, which will enable host and donor cells to be distinguished from each other after keratolimbal allografting procedures, even if the host and donor are the same sex or siblings. These DNA fingerprinting methods allow assessment of quality and quantity of donor cell survival, as well as survival time. The extreme sensitivity and accuracy of the technique means that should contamination occur, it would be identified, thus ensuring meaningful results.
Authors:
T R Henderson; I Findlay; P L Matthews; B A Noble
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cornea     Volume:  20     ISSN:  0277-3740     ISO Abbreviation:  Cornea     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-02     Completed Date:  2001-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8216186     Medline TA:  Cornea     Country:  United States    
Other Details:
Languages:  eng     Pagination:  400-3     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Leeds General Infirmary, Leeds, U.K.
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MeSH Terms
Descriptor/Qualifier:
Cell Transplantation*
DNA / analysis
DNA Fingerprinting / methods*
Epithelial Cells / transplantation*
Epithelium, Corneal / cytology*
Humans
Limbus Corneae / cytology*
Phenotype
Polymerase Chain Reaction
Stem Cell Transplantation
Stem Cells / cytology
Tandem Repeat Sequences
Tissue Donors
Transplantation, Homologous
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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