Document Detail


Identifying Multiple Populations from Single-Molecule Lifetime Distributions.
MedLine Citation:
PMID:  23255328     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A major advantage of single-molecule methods over ensemble-averaging techniques involves the ability to characterize heterogeneity through the identification of multiple molecular populations. It can be challenging, however, to determine absolute values of dynamic parameters (and to relate these values to those determined from a conventional method) because characteristic timescales of various populations may vary over many orders of magnitude, and under a given set of experimental conditions instrumental sensitivity to various populations may be unequal. Using data obtained from the single-molecule tracking microscopy of fibrinogen protein adsorption and desorption, it is shown that by performing a combined analysis of molecular trajectories obtained using a range of acquisition times, it is possible to extract quantitative absolute values of multiple population fractions and residence times (with well-defined uncertainties), even when these values span many orders of magnitude. In particular, as many as six distinct populations are rigorously identified, exhibiting characteristic timescales that vary over nearly three orders of magnitude with population fractions as small as one part in a thousand. This approach will lead to better comparability between single-molecule experiments and may be useful in connecting single-molecule to ensemble-averaged observations.
Authors:
Mark Kastantin; Daniel K Schwartz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-18
Journal Detail:
Title:  Chemphyschem : a European journal of chemical physics and physical chemistry     Volume:  -     ISSN:  1439-7641     ISO Abbreviation:  Chemphyschem     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954211     Medline TA:  Chemphyschem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 (USA).
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