| Identification of young megakaryocytes by immunofluorescence and cytophotometry. | |
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MedLine Citation:
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PMID: 361105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The DNA-content of fluoresceine-labeled platelet antigen containing cells of mouse bone marrow was measured. For immunofluorescence highly specific anti-mouse-platelet-serum and fluoresceine-conjugated antigammaglobuline was used, applying the "sandwich" technique. Three hundred panoptically identifable megakaryocytes served as control group. The DNA-polyploidization pattern of megakaryocytes and immunofluorescence positive cells was almost identical. However, among the immunofluorescence positive cells a considerable amount of cells showed DNA-values lower than 4c, whereas the megakaryocytes of the Pappenheim stained smears revealed no DNA-values lower than 4c. The percentages of diploid and tetraploid cells, respectively, was 6 and 7% compared with 0 and 1% of panoptically identifiable megakaryoctyes. The results suggest that young megakaryocytic cells with diploid and tetraploid DNA-values can be detected by immunofluorescence technique, indicating that the flow from the uncommited to the committed megakaryocytic precursor cell appears at this early stage of megakaryocyte production. |
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Authors:
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M Mayer; J Schaefer; W Queisser |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Blut Volume: 37 ISSN: 0006-5242 ISO Abbreviation: Blut Publication Date: 1978 Nov |
Date Detail:
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Created Date: 1979-01-24 Completed Date: 1979-01-24 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0173401 Medline TA: Blut Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 265-70 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals DNA / analysis Diploidy Fluorescent Antibody Technique Male Megakaryocytes / cytology* Mice Photometry Polyploidy Rabbits / immunology |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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