Document Detail


Identification of a xylose-containing cerebroside in the salt gland of the herring gull.
MedLine Citation:
PMID:  4335795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A pentose-containing cerebroside has been identified in the salt gland of the herring gull, using mass spectrometry of acetyl and trimethylsilyl derivatives. A detailed interpretation of the spectra allowed a conclusion concerning the major long-chain base (the C(20) homolog of sphingosine) and the major fatty acids (C(22)-C(25) 2-hydroxy fatty acids), using reference spectra of synthetic galactosylceramides. A six-membered glycose ring (aldopyranose) was demonstrated by mass spectrometry of the acetyl derivative of periodic acid-oxidized and sodium borodeuteride-reduced pentosylceramide. By gas-liquid chromatography and mass spectrometry of methanolysis products, the pentose was shown to be identical with xylose. The procedures were applied to 25-50 micro g of glycolipid.
Authors:
K A Karlsson; B E Samuelsson; G O Steen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  13     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1972 Mar 
Date Detail:
Created Date:  1972-06-13     Completed Date:  1972-06-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Birds
Borohydrides
Cerebrosides / analysis*,  isolation & purification
Chemical Phenomena
Chemistry
Chromatography, Gas
Chromatography, Thin Layer
Deuterium
Fatty Acids / analysis
Fatty Alcohols / analysis
Mass Spectrometry
Models, Chemical
Oxidation-Reduction
Periodic Acid
Salt Gland / analysis*
Xylose / analysis
Chemical
Reg. No./Substance:
0/Borohydrides; 0/Cerebrosides; 0/Fatty Acids; 0/Fatty Alcohols; 0/Xylose; 10450-60-9/Periodic Acid; 7782-39-0/Deuterium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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