Document Detail

Identification of viral genes required for cell-to-cell movement of southern bean mosaic virus.
MedLine Citation:
PMID:  9878617     Owner:  NLM     Status:  MEDLINE    
Inoculation of Vigna unguiculata (cowpea) with transcripts synthesized in vitro from a genome-length cDNA clone of the cowpea strain of southern bean mosaic virus (SBMV-C) resulted in a systemic SBMV-C infection of this host. Capped RNA was about five times more infectious than uncapped RNA as determined by a local lesion assay. The SBMV-C cDNA clone was also used for mutagenesis of the four SBMV-C open reading frames (ORFs). ORF1, ORF3, and coat protein (CP) mutants were not infectious in cowpea. Electroporation of cowpea protoplasts with mutant transcripts demonstrated that the ORF1, ORF3, and CP gene products were not required for SBMV-C RNA synthesis, and the ORF1 and ORF3 gene products were not required for SBMV-C assembly. From these results, it was concluded that the ORF1 and ORF3 proteins and the CP are required for SBMV-C cell-to-cell movement. One of the ORF3 mutants pSBMV2-UAA1833 contained a nonsense codon between the predicted -1 ribosomal frameshift site (SBMV-C nucleotides 1796-1802) and a potential ORF3 translation initiation codon at SBMV-C nucleotide 1895. The lack of infectivity of this mutant suggested that ORF3 was expressed by a -1 ribosomal frameshift in ORF2 rather than by initiation of translation at nucleotide 1895.
K Sivakumaran; B C Fowler; D L Hacker
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  252     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-01-28     Completed Date:  1999-01-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  376-86     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Department of Microbiology and Center for Legume Research, University of Tennessee, Knoxville, Tennessee, 37996-0845, USA.
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MeSH Terms
Fabaceae / virology*
Genome, Viral
Mosaic Viruses / genetics,  pathogenicity,  physiology*
Mutagenesis, Site-Directed
Open Reading Frames*
Plant Viral Movement Proteins
Plants, Medicinal*
Protoplasts / virology
RNA, Viral / genetics,  isolation & purification
Transcription, Genetic
Viral Proteins / genetics,  physiology
Grant Support
Reg. No./Substance:
0/Plant Viral Movement Proteins; 0/RNA, Viral; 0/Viral Proteins

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