Document Detail


Identification of an upstream activation site in the pyruvate decarboxylase structural gene (PDC1) of Saccharomyces cerevisiae.
MedLine Citation:
PMID:  3147147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The upstream region of the Saccharomyces cerevisiae pyruvate decarboxylase structural gene, PDC1, has been isolated and fused to the indicator gene Escherichia coli lacZ. 1.2 kb of the upstream region has been sequenced. The PDC1-lacZ fusion has been integrated at the ura3-52 locus in the yeast genome, and has a basal level of expression on ethanol. On glucose media this level is increased 30-50 fold. An upstream activation site, UASpdc, between 793 and 535 bp upstream from the ATG of PDC1, which mediates the response to glucose has been identified by deletion analysis. The UASpdc contains a consensus RPG box, originally identified in ribosomal protein genes (Leer et al. 1985). The function of UASpdc is independent of distance from the ATG. There is also an upstream repressing sequence located between 535 and 385 bp upstream from the translational start of PDC1.
Authors:
G Butler; D J McConnell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current genetics     Volume:  14     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-03-29     Completed Date:  1989-03-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  405-12     Citation Subset:  IM    
Affiliation:
Department of Genetics, Trinity College, Dublin, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Carboxy-Lyases / genetics*
Cloning, Molecular
DNA, Fungal
Endodeoxyribonucleases
Gene Expression Regulation
Genes*
Glucose / pharmacology
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic
Pyruvate Decarboxylase / genetics*
Regulatory Sequences, Nucleic Acid*
Restriction Mapping
Saccharomyces cerevisiae / enzymology,  genetics*
Transformation, Genetic
beta-Galactosidase / genetics
Chemical
Reg. No./Substance:
0/DNA, Fungal; 50-99-7/Glucose; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.11.-/exonuclease Bal 31; EC 3.2.1.23/beta-Galactosidase; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.1/Pyruvate Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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