Document Detail

Identification of an unusual cyclooxygenase metabolite of arachidonic acid in rabbit renal medulla.
MedLine Citation:
PMID:  6437329     Owner:  NLM     Status:  MEDLINE    
A renal medulla 100,000g pellet metabolized arachidonic acid, C20:4, to the previously described prostaglandins prostaglandin E2, 6-ketoprostaglandin F1 alpha, thromboxane B2, 12-hydroxyheptadecatrienoic acid, and 11-hydroxyeicosatetraenoic acid. In addition, under conditions of low enzyme to substrate ratios, the renal medulla also produced an unusual metabolite from arachidonic acid. This metabolite was inhibited by indomethacin, and thus suggested that it was a product of the cyclooxygenase. Addition of GSH to the incubation inhibited its formation, while p-hydroxymercuribenzoate enhanced its formation. This compound was identified by HPLC purification, uv absorption, and gas chromatography-mass spectroscopy. The compound was 9,15-dioxo,11-hydroxyprosta-5,13-dienoic acid.
A R Morrison; J R Crowley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  234     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-12-05     Completed Date:  1984-12-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  413-7     Citation Subset:  IM    
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MeSH Terms
Arachidonic Acid
Arachidonic Acids / metabolism*
Dinoprostone* / analogs & derivatives*
Fatty Acids, Unsaturated / biosynthesis
Glutathione / pharmacology
Hydroxyeicosatetraenoic Acids / biosynthesis
Indomethacin / pharmacology
Kidney Medulla / enzymology*
Prostaglandin-Endoperoxide Synthases / metabolism*
Prostaglandins / biosynthesis
Prostaglandins E / biosynthesis*
Thromboxane B2 / biosynthesis
Grant Support
R0130542//PHS HHS; RR00954/RR/NCRR NIH HHS
Reg. No./Substance:
0/Arachidonic Acids; 0/Fatty Acids, Unsaturated; 0/Hydroxyeicosatetraenoic Acids; 0/Prostaglandins; 0/Prostaglandins E; 26441-05-4/15-ketoprostaglandin E2; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin; 54397-85-2/Thromboxane B2; 70-18-8/Glutathione; 73151-66-3/11-hydroxy-5,8,12,14-eicosatetraenoic acid; 76448-34-5/12-hydroxy-4,8,10-heptadecatrienoic acid; EC Synthases

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