Document Detail

Identification of two new D variants, DHMi and DHMii using monoclonal anti-D.
MedLine Citation:
PMID:  8578737     Owner:  NLM     Status:  MEDLINE    
Fifteen examples of red cells are described which showed discrepant reactivity on routine grouping with two monoclonal anti-D typing reagents, HAM-A being negative and MAD-2 strongly positive. The reaction profile of these cells with a number of monoclonal anti-D characterised the samples into two new variant groups, DHMi and DHMii. DHMi was characterised by a negative reaction with BRAD-8 and DHMii by a negative reaction with BRAD-5. DHMi cells were found to have a papain-sensitive site, identified by Mab 21 G6, which was not detectable in DHMii. The presence of allo-anti-D in one case of DHMi suggests a partial D in these individuals. The positive reaction of HAM-A with sialidase- and endo-F-treated DHMii cells suggests that sialic acid and/or N-glycans could possibly be involved in blocking the reaction with untreated cells. All samples typed as C-E+. One was e negative while the rest had variable depression of the e and f antigens. Marginal depression of E was seen with those DHMi cells tested, but not with DHMii cells. Data from family studies suggest that the variant D in both DHMi and DHMii is inherited as a cDE gene complex and is controlled by the RH locus.
J Jones
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Vox sanguinis     Volume:  69     ISSN:  0042-9007     ISO Abbreviation:  Vox Sang.     Publication Date:  1995  
Date Detail:
Created Date:  1996-03-13     Completed Date:  1996-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413606     Medline TA:  Vox Sang     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  236-41     Citation Subset:  IM    
International Blood Group Reference Laboratory, Bristol, UK.
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MeSH Terms
Antibodies, Monoclonal
Antigenic Variation / genetics*
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Peptides / isolation & purification*
Rh-Hr Blood-Group System / genetics,  isolation & purification*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Peptides; 0/Rh-Hr Blood-Group System; 0/Rho(D) antigen; EC; EC 3.5.-/Amidohydrolases; EC Asparagine Amidase

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