Document Detail

Identification of two isoforms of the vesicle-membrane fusion protein SNAP-23 in human neutrophils and HL-60 cells.
MedLine Citation:
PMID:  9070898     Owner:  NLM     Status:  MEDLINE    
We have identified and cloned two isoforms of SNAP-23 in human neutrophils and human promyelocytic HL-60 cells. SNAP-23A which is similar to the recently cloned SNAP-23, and a novel isoform, termed SNAP-23B. SNAP-23B results from a deletion of 159 bp of the SNAP-23A cDNA, encoding a protein of approximately 17.8 kDa. SNAP-23B is identical to SNAP-23A, but lacks fifty-three amino acid residues, from 90 to 142. This region of SNAP-23A has a target sequence for posttranslational fatty acid acylation, suggesting that the two isoforms can differ in their capacity to interact with membranes. The level of SNAP-23 mRNA is increased during HL-60 neutrophil differentiation. These findings demonstrate the existence of two SNAP-23 proteins in human neutrophils; and therefore, these cells have all the proteins needed to carry out the exocytic process.
F Mollinedo; P A Lazo
Related Documents :
7735128 - Cerebrin-50, a human cerebrospinal fluid protein whose mrna is present in multiple tiss...
1722468 - Molecular cloning and expression of the cdna coding for a new member of the s100 protei...
19322678 - Partial molecular characterization, expression pattern, polymorphism and association an...
21795388 - Kinesin molecular motor eg5 functions during polypeptide synthesis.
17179418 - Characterization of the chicken small intestine type iib sodium phosphate cotransporter.
7993968 - Cloning and high level expression of gene encoding es antigen from trichinella spiralis...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  231     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-04-16     Completed Date:  1997-04-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  808-12     Citation Subset:  IM    
Laboratory of Leukocyte Biology, Facultad de Medicina, Consejo Superior de Investigaciones Científicas-Universidad de Valladolid, C/Ramón y Cajal, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Y09567;  Y09568
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Carrier Proteins / chemistry,  metabolism*
Cell Degranulation
HL-60 Cells / metabolism*
Membrane Fusion*
Molecular Sequence Data
Neutrophils / metabolism*
Qb-SNARE Proteins
Qc-SNARE Proteins
Sequence Alignment
Reg. No./Substance:
0/Carrier Proteins; 0/Qb-SNARE Proteins; 0/Qc-SNARE Proteins; 0/SNAP23 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  L-selectin regulates actin polymerisation via activation of the small G-protein Rac2.
Next Document:  An optimized protocol for mRNA quantification using nested competitive RT-PCR.