Document Detail


Identification of two distinct Bacillus subtilis citrate synthase genes.
MedLine Citation:
PMID:  8045898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two distinct Bacillus subtilis genes (citA and citZ) were found to encode citrate synthase isozymes that catalyze the first step of the Krebs cycle. The citA gene was cloned by genetic complementation of an Escherichia coli citrate synthase mutant strain (W620) and was in a monocistronic transcriptional unit. A divergently transcribed gene, citR, could encode a protein with strong similarity to the bacterial LysR family of regulatory proteins. A null mutation in citA had little effect on citrate synthase enzyme activity or sporulation. The residual citrate synthase activity was purified from a citA null mutant strain, and the partial amino acid sequence for the purified protein (CitZ) was determined. The citZ gene was cloned from B. subtilis chromosomal DNA by using a PCR-generated probe synthesized with oligonucleotide primers derived from the partial amino acid sequence of purified CitZ. The citZ gene proved to be the first gene in a tricistronic cluster that also included citC (coding for isocitrate dehydrogenase) and citH (coding for malate dehydrogenase). A mutation in citZ caused a substantial loss of citrate synthase enzyme activity, glutamate auxotrophy, and a defect in sporulation.
Authors:
S Jin; A L Sonenshein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  176     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-08-30     Completed Date:  1994-08-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4669-79     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U05256;  U05257
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MeSH Terms
Descriptor/Qualifier:
Aconitate Hydratase / metabolism
Amino Acid Sequence
Bacillus subtilis / enzymology,  genetics*
Bacterial Proteins / genetics
Base Sequence
Chromosome Mapping
Citrate (si)-Synthase / genetics*
Cloning, Molecular
Genes, Bacterial / genetics*
Genetic Complementation Test
Isocitrate Dehydrogenase / genetics
Isoenzymes / genetics*
Malate Dehydrogenase / genetics
Molecular Sequence Data
Multigene Family / genetics
Mutagenesis
Sequence Deletion
Spores, Bacterial / growth & development
Transcription Factors / genetics
Transcription, Genetic
Transformation, Genetic
Grant Support
ID/Acronym/Agency:
GM36718/GM/NIGMS NIH HHS; GM42219/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Isoenzymes; 0/Transcription Factors; 87609-37-8/LysR protein, Bacteria; EC 1.1.1.37/Malate Dehydrogenase; EC 1.1.1.41/Isocitrate Dehydrogenase; EC 2.3.3.1/Citrate (si)-Synthase; EC 4.2.1.3/Aconitate Hydratase
Comments/Corrections

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