Document Detail


Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species.
MedLine Citation:
PMID:  21106037     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
• In this study, six putative promoter regions of phosphate transporter Pht1;3, Pht1;4 and Pht1;5 genes were isolated from eggplant and tobacco using the inverse polymerase chain reaction (iPCR). The isolated sequences show evolutionary conservation and divergence within/between the two groups of Pht1;3 and Pht1;4/Pht1;5. • Histochemical analyses showed that all six promoter fragments were sufficient to drive β-glucuronidase (GUS) expression specifically in arbuscular mycorrhizal (AM) tobacco roots and were confined to distinct cells containing AM fungal structures (arbuscules or intracellular hyphae). • A series of promoter truncation and mutation analyses combined with phylogenetic footprinting of these promoters revealed that at least two cis-regulatory elements - the mycorrhiza transcription factor binding sequence (MYCS) first identified in this study and P1BS - mediated the transcriptional activation of the AM-mediated inorganic phosphate (Pi) transporter genes. Deletion or partial mutation of either of the two motifs in the promoters could cause a remarkable decrease, or even complete absence, of the promoter activity. • Our results propose that uptake of inorganic phosphate (Pi) by AM fungi is regulated, at least partially, in an MYCS- and P1BS-dependent manner in eudicot species. Our finding offers new insights into the molecular mechanisms underlying the coordination between the AM and the Pi signalling pathways.
Authors:
Aiqun Chen; Mian Gu; Shubin Sun; Lingling Zhu; Shuai Hong; Guohua Xu
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Publication Detail:
Type:  Journal Article     Date:  2010-11-25
Journal Detail:
Title:  The New phytologist     Volume:  189     ISSN:  1469-8137     ISO Abbreviation:  New Phytol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882884     Medline TA:  New Phytol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1157-69     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. New Phytologist © 2010 New Phytologist Trust.
Affiliation:
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
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