| Identification of two additives, locust bean gum (E-410) and guar gum (E-412), in food products by DNA-based methods. | |
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MedLine Citation:
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PMID: 15370834 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Locust bean gum (E-410) and guar gum (E-412) are high molecular weight galactomannans used by the food industry as versatile food additives. The compounds, although chemically closely related, do not have the same functional properties when used in foods, and the substitution or unadvertised addition of either could change the desired qualities of the product. Analytical discrimination between E-410 and E-412 is technically difficult since they only differ in their galactose: mannose ratios, being 1 : 4 and 1 : 2 for locust bean gum and guar gum, respectively. A qualitative DNA-based method is reported for the authentication of additives E-410 and E-412 in finished food products (ice cream, dehydrated desserts, milk derivatives, dehydrated soups, salad dressing, marmalade and meat) from small quantities of food. DNA sequences from the nuclear ribosomal spacers of Ceratonia siliqua and Cyamopsis tetragonoloba, the plant sources of E-410 and E-412, respectively, were used to design polymerase chain reaction primers specific for each additive (PA23/PA21 and PG22/PG21). Twenty-two foods were analysed for the presence of E-410 and E-412 additives by this single-step polymerase chain reaction-based method. Positive DNA amplifications with the E-410 and/or E-412 primers were obtained in all 19 samples reported to contain either additive. |
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Authors:
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M Urdiain; A Doménech-Sánchez; S Albertí; V J Benedí; J A Rosselló |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Food additives and contaminants Volume: 21 ISSN: 0265-203X ISO Abbreviation: Food Addit Contam Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-09-16 Completed Date: 2005-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8500474 Medline TA: Food Addit Contam Country: England |
Other Details:
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Languages: eng Pagination: 619-25 Citation Subset: IM |
Affiliation:
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Microbiología, Departament de Biología, Facultad de Ciencias, Universitat de les Illes Balears and IMEDEA (CSIC-UIB), E-07071 Palma de Mallorca, Spain. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cheese / analysis DNA, Plant / analysis* DNA, Ribosomal Spacer / analysis Food Additives / analysis* Food Analysis / methods* Galactans / analysis* Genetic Markers Ice Cream / analysis Mannans / analysis* Meat / analysis Milk / chemistry Nucleic Acid Amplification Techniques / methods Plant Gums Polymerase Chain Reaction / methods Polysaccharides / analysis* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Plant; 0/DNA, Ribosomal Spacer; 0/Food Additives; 0/Galactans; 0/Genetic Markers; 0/Mannans; 0/Plant Gums; 0/Polysaccharides; 9000-30-0/guar gum; 9000-40-2/locust bean gum |
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