Document Detail

Identification of two additives, locust bean gum (E-410) and guar gum (E-412), in food products by DNA-based methods.
MedLine Citation:
PMID:  15370834     Owner:  NLM     Status:  MEDLINE    
Locust bean gum (E-410) and guar gum (E-412) are high molecular weight galactomannans used by the food industry as versatile food additives. The compounds, although chemically closely related, do not have the same functional properties when used in foods, and the substitution or unadvertised addition of either could change the desired qualities of the product. Analytical discrimination between E-410 and E-412 is technically difficult since they only differ in their galactose: mannose ratios, being 1 : 4 and 1 : 2 for locust bean gum and guar gum, respectively. A qualitative DNA-based method is reported for the authentication of additives E-410 and E-412 in finished food products (ice cream, dehydrated desserts, milk derivatives, dehydrated soups, salad dressing, marmalade and meat) from small quantities of food. DNA sequences from the nuclear ribosomal spacers of Ceratonia siliqua and Cyamopsis tetragonoloba, the plant sources of E-410 and E-412, respectively, were used to design polymerase chain reaction primers specific for each additive (PA23/PA21 and PG22/PG21). Twenty-two foods were analysed for the presence of E-410 and E-412 additives by this single-step polymerase chain reaction-based method. Positive DNA amplifications with the E-410 and/or E-412 primers were obtained in all 19 samples reported to contain either additive.
M Urdiain; A Doménech-Sánchez; S Albertí; V J Benedí; J A Rosselló
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food additives and contaminants     Volume:  21     ISSN:  0265-203X     ISO Abbreviation:  Food Addit Contam     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-09-16     Completed Date:  2005-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500474     Medline TA:  Food Addit Contam     Country:  England    
Other Details:
Languages:  eng     Pagination:  619-25     Citation Subset:  IM    
Microbiología, Departament de Biología, Facultad de Ciencias, Universitat de les Illes Balears and IMEDEA (CSIC-UIB), E-07071 Palma de Mallorca, Spain.
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MeSH Terms
Base Sequence
Cheese / analysis
DNA, Plant / analysis*
DNA, Ribosomal Spacer / analysis
Food Additives / analysis*
Food Analysis / methods*
Galactans / analysis*
Genetic Markers
Ice Cream / analysis
Mannans / analysis*
Meat / analysis
Milk / chemistry
Nucleic Acid Amplification Techniques / methods
Plant Gums
Polymerase Chain Reaction / methods
Polysaccharides / analysis*
Reg. No./Substance:
0/DNA, Plant; 0/DNA, Ribosomal Spacer; 0/Food Additives; 0/Galactans; 0/Genetic Markers; 0/Mannans; 0/Plant Gums; 0/Polysaccharides; 9000-30-0/guar gum; 9000-40-2/locust bean gum

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