Document Detail

Identification of tumour initiating cells in feline head and neck squamous cell carcinoma and evidence for gefitinib induced epithelial to mesenchymal transition.
MedLine Citation:
PMID:  22342216     Owner:  NLM     Status:  MEDLINE    
Feline oral squamous cell carcinoma is considered a highly invasive cancer that carries a high level of morbidity. Despite aggressive surgery, patients often succumb to disease, the tumour having inherent insensitivity to radiation and chemotherapy. In this study we sought to identify cells within the feline SCC1 line that have stem cell properties, including inherent resistance mechanisms. When feline cells were subjected to harsh growth conditions, they formed sphere colonies consistent with a stem cell phenotype. Utilising CD133, we were able to identify a small fraction of cells within the population that had enhanced sphere-forming ability, reduced sensitivity to radiation and conventional chemotherapy and demonstrated resistance to the EGFR-targeting drug, gefitinib. In addition, long-term culture of feline SSC1 cells in gefitinib caused a change in cell morphology and gene expression reminiscent of an epithelial to mesenchymal transition. Taken together, these results suggest that feline SCC may be driven by small subset of cancer stem cells.
L Y Pang; G T Bergkvist; A Cervantes-Arias; D A Yool; R Muirhead; D J Argyle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-17
Journal Detail:
Title:  Veterinary journal (London, England : 1997)     Volume:  193     ISSN:  1532-2971     ISO Abbreviation:  Vet. J.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-10     Completed Date:  2012-11-19     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9706281     Medline TA:  Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  46-52     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Royal (Dick) School of Veterinary Studies and Roslin Institute, The University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
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MeSH Terms
Antigens, CD / metabolism
Antineoplastic Agents / pharmacology
Carcinoma, Squamous Cell / drug therapy*,  radiotherapy*
Cat Diseases / drug therapy*,  radiotherapy*
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Transformation, Neoplastic
Drug Resistance, Neoplasm
Epithelial-Mesenchymal Transition
Glycoproteins / metabolism
Head and Neck Neoplasms / drug therapy*,  radiotherapy*
Neoplastic Stem Cells / cytology*,  drug effects,  radiation effects
Peptides / metabolism
Quinazolines / pharmacology
Receptor, Epidermal Growth Factor / antagonists & inhibitors
Signal Transduction
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Antineoplastic Agents; 0/Glycoproteins; 0/Peptides; 0/Quinazolines; EC, Epidermal Growth Factor; S65743JHBS/gefitinib
Comment In:
Vet J. 2012 Jul;193(1):6-7   [PMID:  22498785 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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