Document Detail


Identification of transcription factors required for the expression of mammalian U6 genes in vitro.
MedLine Citation:
PMID:  1868835     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcription factors, required for the basal expression of the mouse U6 gene were identified in extracts from HeLa cells. This gene is transcribed at least four times more efficiently than its human counterpart in extracts from mouse or HeLa cells and hence provides an excellent in vitro system for the identification of transcription factors involved in the basal expression of mammalian U6 genes. At least four separate protein components were found to be required in addition to RNA polymerase III for correct synthesis of U6 RNA in vitro. These correspond to: (i) TFIIIB; (ii) a heat labile activity contained in a protein fraction enriched in TFIID; (iii) an, as yet, uncharacterized component contained in the flow-through upon rechromatography on phosphocellulose, and finally; (iv) a protein specifically binding to the mouse U6 gene promoter and transactivating its expression. Transcription factors IIIA and IIIC are not involved in mammalian U6 transcription in vitro. The U6-specific transcription factor has a molecular mass of approximately 90 +/- 10 kDa. It specifically binds to the U6 gene from bp -42 to -78 on the coding and from bp -37 to -79 on the non-coding strand thereby centrally encompassing the PSE motif of the mouse U6 promoter. The binding activity of this protein is correlated with the efficiency with which the U6 gene is transcribed in vitro, thereby indicating a crucial role of the PSE-binding protein for U6 transcription.
Authors:
R Waldschmidt; I Wanandi; K H Seifart
Related Documents :
23677445 - Silencing of pancreatic adenocarcinoma upregulated factor by rna interference inhibits ...
23149195 - Differentiation of rat dermal papilla cells into fibroblast-like cells induced by trans...
25051915 - Ephb4 regulates the growth and migration of pancreatic cancer cells.
24321385 - Cohesin and ctcf differentially regulate spatiotemporal runx1 expression during zebrafi...
19361795 - Imprinting: rna expression for homocysteine recycling in the human oocyte.
12370805 - Both the smad and p38 mapk pathways play a crucial role in runx2 expression following i...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  10     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-09-18     Completed Date:  1991-09-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2595-603     Citation Subset:  IM    
Affiliation:
Institut für Molekularbiologie und Tumorforschung, Marburg, FRG.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Electrophoresis, Polyacrylamide Gel
Gene Expression*
Hela Cells
Humans
Mice
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic
RNA, Small Nuclear / genetics*,  metabolism
Transcription Factor TFIID
Transcription Factor TFIIIA
Transcription Factor TFIIIB
Transcription Factors / isolation & purification,  metabolism*
Transcription Factors, TFIII*
Chemical
Reg. No./Substance:
0/RNA, Small Nuclear; 0/Transcription Factor TFIID; 0/Transcription Factor TFIIIA; 0/Transcription Factor TFIIIB; 0/Transcription Factors; 0/Transcription Factors, TFIII; 0/transcription factor TFIIIC
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Flexibility of the DNA enhances promoter affinity of Escherichia coli RNA polymerase.
Next Document:  Short leader sequences may be transferred from small RNAs to pre-mature mRNAs by trans-splicing in E...