Document Detail


Identification of tissue-specific vasculogenic cells originating from murine uterus.
MedLine Citation:
PMID:  16311738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endometrium is a highly regenerative adult tissue that undergoes repeated degeneration and regeneration following menarche. Therefore, it is believed that endometrium contains stem and/or progenitor cells in order to compensate for the regeneration of tissue components. We report here that stem-like cells having vasculogenic potential are present in the uterus. Enzymatically extracted cells from murine uteri were characterized and fractionated into four subpopulations by flowcytometry; CD34(+)/45(-) (Ut-34), CD34(-)/45(-) (Ut-DN) and the remaining CD45(+) cell fractions (CD34(+)/45(+) and CD34(-)/45(+) cells). The Ut-34 and Ut-DN fractions were mostly negative for putative endothelial cell (EC) markers, such as CD31, Flk-1, c-kit and VE-cadherin, although the Ut-DN fraction contained 2.8% CD31(+) cells. Ut-DN cells were further divided into CD31(+) and CD31(-) fractions. Three cell populations were obtained from green fluorescence protein (GFP) transgenic mice and were transplanted into injured wild-type mouse skeletal muscle. At 4 weeks after cell transplantation, donor-derived vascular smooth muscle and ECs were observed in the injured recipient muscle. A similar trend was observed in the Ut-34 group, but differentiation into vascular smooth muscle was predominant. In contrast, the Ut-DN/31(+) cell-transplanted group showed preferential differentiation into vascular ECs, thus suggesting that they were relatively committed preexisting ECs. These characteristics were also seen in vitro, in clonal cell cultures. Interestingly, donor derived Ut-DN/31(+), Ut-DN/31(-) and Ut-34 cells could not be identified after bone marrow (BM) transplantation, thus confirming that they are not derived from BM. It therefore appeared that tissue-specific vasculogenic cells are present in the murine uterus and that they exhibit vascular formation, even in different tissue microenvironments.
Authors:
Narumi Onodera; Tetsuro Tamaki; Yoshinori Okada; Akira Akatsuka; Daisuke Aoki
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Publication Detail:
Type:  Journal Article     Date:  2005-11-26
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  125     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-19     Completed Date:  2007-05-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  625-35     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Keio University School of Medicine, Shinanomachi 35, Shinjuku, 160-8582, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / analysis,  genetics
Bone Marrow Cells / chemistry
Cell Differentiation*
Cell Separation
Endothelium, Vascular / chemistry,  cytology*
Female
Green Fluorescent Proteins / analysis,  genetics
Mice
Mice, Transgenic
Muscle Cells / chemistry
Myocytes, Smooth Muscle / chemistry
Neovascularization, Physiologic*
RNA, Messenger / analysis,  metabolism
Stem Cells / chemistry,  cytology*
Uterus / blood supply*,  cytology*
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/RNA, Messenger; 147336-22-9/Green Fluorescent Proteins

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